Galibert F, Chen T N, Mandart E
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5315-9. doi: 10.1073/pnas.78.9.5315.
Nucleotide sequence analysis was performed with the Maxam--Gilbert method on a cloned woodchuck hepatitis virus DNA (Eco WHV DNA). The structural gene coding for the envelope protein of the virus was localized on the viral genome in the partially single-stranded region between map positions 91.2 and 71. This gene is composed of 669 nucleotides and can code for a polypeptide of 25,645 daltons. The DNA sequence and the deduced amino acid sequence were compared with those of the corresponding gene and surface antigen of the related hepatitis B virus, allowing some insight into the localization of the antigenic site.
采用马克萨姆-吉尔伯特法对克隆的土拨鼠肝炎病毒DNA（Eco WHV DNA）进行了核苷酸序列分析。编码该病毒包膜蛋白的结构基因定位在病毒基因组中位于图谱位置91.2和71之间的部分单链区域。该基因由669个核苷酸组成，可编码一个25,645道尔顿的多肽。将该DNA序列和推导的氨基酸序列与相关乙型肝炎病毒的相应基因和表面抗原的序列进行了比较，从而对抗原位点的定位有了一些了解。
