Wuthier P, Jones C, Puck T T
J Exp Med. 1973 Jul 1;138(1):229-44. doi: 10.1084/jem.138.1.229.
A second surface antigen, B(L), lethal in the presence of specific antibody and complement has been identified on some human cells and shown to behave as a good genetic marker. It is autosomal, unlinked to the human A(L) antigen previously described, and unlinked to 15 other human genes. The A(L) antigen, which is linked to the lactic dehydrogenase A gene, is found on the HeLa, the cultured human fibroblast, and in small amounts on the human lymphocyte. B(L) occurs on HeLa cells, on cultured human fibroblasts, and on human lymphocytes, but not on human RBCs. Hybrid cells formed by fusion of human and Chinese hamster cells have been prepared containing each of the four possible combinations of these two markers. Highly selective antisera sensitive to each marker separately can be obtained. The use of single-cell plating to demonstrate the presence of the antigens and of hybrid cells containing desired combinations of the markers facilitates study in this system.
第二种表面抗原B(L)已在一些人类细胞上被鉴定出来，在存在特异性抗体和补体的情况下具有致死性，并被证明是一种良好的遗传标记。它是常染色体的，与先前描述的人类A(L)抗原不连锁，也与其他15个人类基因不连锁。与乳酸脱氢酶A基因连锁的A(L)抗原存在于HeLa细胞、培养的人类成纤维细胞中，在人类淋巴细胞中含量较少。B(L)存在于HeLa细胞、培养的人类成纤维细胞和人类淋巴细胞上，但不存在于人类红细胞上。已经制备了由人类和中国仓鼠细胞融合形成的杂交细胞，其中包含这两种标记的四种可能组合中的每一种。可以获得分别对每种标记敏感的高度选择性抗血清。使用单细胞平板培养来证明抗原的存在以及含有所需标记组合的杂交细胞，便于对该系统进行研究。
