通过改良的¹²⁵I标记DNA结合试验测定抗DNA抗体。使用阴离子去污剂消除DNA与非免疫球蛋白碱性蛋白的非特异性结合。
Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent.
作者信息
Izui S, Lambert P H, Miescher P A
出版信息
Clin Exp Immunol. 1976 Dec;26(3):425-30.
Abstract
A modified 125I-labelled DNA-binding test was developed. By using an anionic detergent, 0-025% sodium dodecyl sulphate, the non-specific binding of DNA by basic proteins was completely eliminated, while the specific binding of DNA by anti-DNA antibodies was not interfered with. The level of DNA-binding activity in normal human sera was decreased in the modified DNA-binding test, while its sensitivity was similar to that of the Farr assay in presence of sera from SLE patients.
摘要
开发了一种改良的¹²⁵I标记DNA结合试验。通过使用阴离子去污剂0.025%的十二烷基硫酸钠，碱性蛋白与DNA的非特异性结合被完全消除，而抗DNA抗体与DNA的特异性结合未受干扰。在改良的DNA结合试验中，正常人血清中的DNA结合活性水平降低，而在存在系统性红斑狼疮患者血清的情况下，其敏感性与Farr试验相似。
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