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通过改良的¹²⁵I标记DNA结合试验测定抗DNA抗体。使用阴离子去污剂消除DNA与非免疫球蛋白碱性蛋白的非特异性结合。

Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent.

作者信息

Izui S, Lambert P H, Miescher P A

出版信息

Clin Exp Immunol. 1976 Dec;26(3):425-30.

PMID:795575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1540975/
Abstract

A modified 125I-labelled DNA-binding test was developed. By using an anionic detergent, 0-025% sodium dodecyl sulphate, the non-specific binding of DNA by basic proteins was completely eliminated, while the specific binding of DNA by anti-DNA antibodies was not interfered with. The level of DNA-binding activity in normal human sera was decreased in the modified DNA-binding test, while its sensitivity was similar to that of the Farr assay in presence of sera from SLE patients.

摘要

开发了一种改良的¹²⁵I标记DNA结合试验。通过使用阴离子去污剂0.025%的十二烷基硫酸钠，碱性蛋白与DNA的非特异性结合被完全消除，而抗DNA抗体与DNA的特异性结合未受干扰。在改良的DNA结合试验中，正常人血清中的DNA结合活性水平降低，而在存在系统性红斑狼疮患者血清的情况下，其敏感性与Farr试验相似。

相似文献

1
Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent.通过改良的¹²⁵I标记DNA结合试验测定抗DNA抗体。使用阴离子去污剂消除DNA与非免疫球蛋白碱性蛋白的非特异性结合。
Clin Exp Immunol. 1976 Dec;26(3):425-30.
2
Expression in systemic lupus erythematosus of an idiotype common to DNA-binding and nonbinding monoclonal antibodies produced by normal human lymphoid cells.正常人淋巴细胞产生的与DNA结合及不结合的单克隆抗体共有的独特型在系统性红斑狼疮中的表达。
J Clin Invest. 1989 Mar;83(3):1002-9. doi: 10.1172/JCI113941.
3
Specific and shared idiotypes found on hybridoma anti-DNA autoantibodies derived from rheumatoid arthritis and systemic lupus erythematosus patients.在源自类风湿性关节炎和系统性红斑狼疮患者的杂交瘤抗DNA自身抗体上发现的特异性和共享独特型。
J Immunol. 1985 Oct;135(4):2385-92.
4
Correlation of DNA-anti-DNA association rates with clinical activity in systemic lupus erythematosus (SLE).系统性红斑狼疮（SLE）中DNA-抗DNA结合率与临床活动的相关性。
J Immunol. 1981 Jan;126(1):16-9.
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Auto-anti-anti-DNA antibodies from SLE patients and normals.来自系统性红斑狼疮患者和正常人的自身抗抗DNA抗体。
J Rheumatol. 1984 Jun;11(3):291-7.
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Spontaneous expression of antibodies to DNA of various species origin in sera of normal subjects and patients with systemic lupus erythematosus.正常人和系统性红斑狼疮患者血清中自发表达的针对各种物种来源DNA的抗体
J Immunol. 1988 Jan 15;140(2):451-5.
7
Comparison of a monoclonal and polyclonal anti-idiotype against a human IgG anti-DNA antibody.一种单克隆抗独特型抗体和一种多克隆抗独特型抗体与一种人IgG抗DNA抗体的比较。
J Autoimmun. 1994 Jun;7(3):349-67. doi: 10.1006/jaut.1994.1025.
8
Detection of masked anti-DNA antibodies in lupus sera by a monoclonal anti-idiotype.利用单克隆抗独特型抗体检测狼疮血清中隐蔽的抗DNA抗体。
J Immunol. 1984 Oct;133(4):1852-6.
9
Detection of immune complexes in unheated sera by modified 125I-Clq binding test. Effect of heating on the binding of Clq by immune complexes and application of the test to systemic lupus erythematosus.用改良的¹²⁵I-Clq结合试验检测未加热血清中的免疫复合物。加热对免疫复合物与Clq结合的影响以及该试验在系统性红斑狼疮中的应用。
J Immunol. 1976 Jan;116(1):232-5.
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[Determination of anti dsDNA antibodies by immunofluorescence using Crithidia luciliae. I--Diagnostic and prognostic value in systemic lupus erythematosus. Comparison with the Farr radioimmunoassay].利用鲁氏锥虫通过免疫荧光法测定抗双链DNA抗体。I——在系统性红斑狼疮中的诊断和预后价值。与法尔放射免疫分析法的比较
Rev Rhum Mal Osteoartic. 1984 Apr;51(4):185-91.

引用本文的文献

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Drug-combination therapy of rheumatoid arthritis.类风湿关节炎的联合药物治疗。
Springer Semin Immunopathol. 1998;20(1-2):309-18. doi: 10.1007/BF00832014.
2
Drug combination therapy of systemic lupus erythematosus.系统性红斑狼疮的联合药物治疗
Springer Semin Immunopathol. 1994;16(2-3):295-311. doi: 10.1007/BF00197523.
3
Low-calorie diet selectively reduces expression of retroviral envelope glycoprotein gp70 in sera of NZB x NZW F1 hybrid mice.低热量饮食选择性降低NZB×NZW F1杂交小鼠血清中逆转录病毒包膜糖蛋白gp70的表达。
J Exp Med. 1981 Oct 1;154(4):1116-24. doi: 10.1084/jem.154.4.1116.
4
Retroviral gp70 immune complexes in NZB x NZW F2 mice with murine lupus nephritis.患有鼠类狼疮性肾炎的NZB×NZW F2小鼠中的逆转录病毒糖蛋白70免疫复合物。
J Exp Med. 1981 Aug 1;154(2):517-28. doi: 10.1084/jem.154.2.517.
5
(NZW x BXSB)F1 hybrid. A model of acute lupus and coronary vascular disease with myocardial infarction.（新西兰白兔×BXSB）F1杂交种。一种伴有心肌梗死的急性狼疮和冠状血管疾病模型。
J Exp Med. 1981 Jul 1;154(1):216-21. doi: 10.1084/jem.154.1.216.
6
Subclass-restricted IgG polyclonal antibody production in mice injected with lipid A-rich lipopolysaccharides.在注射富含脂多糖的小鼠中产生亚类限制性IgG多克隆抗体。
J Exp Med. 1981 Feb 1;153(2):324-38. doi: 10.1084/jem.153.2.324.
7
A spontaneous rheumatoid arthritis-like disease in MRL/l mice.MRL/l小鼠中的一种自发性类风湿性关节炎样疾病。
J Exp Med. 1982 Jun 1;155(6):1690-701. doi: 10.1084/jem.155.6.1690.
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Selective suppression of retroviral gp70-anti-gp70 immune complex formation by prostaglandin E1 in murine systemic lupus erythematosus.前列腺素E1对小鼠系统性红斑狼疮中逆转录病毒gp70-抗gp70免疫复合物形成的选择性抑制作用
J Exp Med. 1980 Dec 1;152(6):1645-58. doi: 10.1084/jem.152.6.1645.
9
Immunological castration of male mice by a totally synthetic vaccine administered in saline.通过在盐水中注射完全合成疫苗对雄性小鼠进行免疫去势。
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5392-5. doi: 10.1073/pnas.79.17.5392.
10
Association of elevated serum glycoprotein gp70 with increased gp70 immune complex formation and accelerated lupus nephritis in autoimmune male BXSB mice.血清糖蛋白gp70升高与自身免疫性雄性BXSB小鼠中gp70免疫复合物形成增加及狼疮性肾炎加速的关联。
Clin Exp Immunol. 1984 May;56(2):272-80.

本文引用的文献

1
Measurement of serum DNA-binding activity in systemic lupus erythematosus.系统性红斑狼疮患者血清DNA结合活性的测定
N Engl J Med. 1969 Sep 25;281(13):701-5. doi: 10.1056/NEJM196909252811304.
2
Antibodies to polynucleotides: distribution in human serums.多核苷酸抗体：在人血清中的分布
Science. 1969 Dec 26;166(3913):1648-9. doi: 10.1126/science.166.3913.1648.
3
Anti-DNA activity in systemic lupus erythematosus. A diagnostic and therapeutic guide.系统性红斑狼疮中的抗DNA活性。诊断与治疗指南。
Ann Rheum Dis. 1971 May;30(3):259-64. doi: 10.1136/ard.30.3.259.
4
Deoxyribonucleic acid antibody: a method to detect its primary interaction with deoxyribonucleic acid.脱氧核糖核酸抗体：一种检测其与脱氧核糖核酸主要相互作用的方法。
Science. 1968 Aug 23;161(3843):806-7. doi: 10.1126/science.161.3843.806.
5
Deoxybonucleic acid (DNA) and antibodies to DNA in the serum of patients with systemic lupus erythematosus.系统性红斑狼疮患者血清中的脱氧核糖核酸（DNA）及抗DNA抗体。
J Clin Invest. 1966 Nov;45(11):1732-40. doi: 10.1172/JCI105479.
6
Iodination of nucleic acids in vitro.核酸的体外碘化
Biochemistry. 1971 May 25;10(11):1993-2000. doi: 10.1021/bi00787a005.
7
Proteins from human cerebrospinal fluid: binding with nucleic acids.来自人类脑脊液的蛋白质：与核酸的结合
Science. 1973 Oct 19;182(4109):296-7. doi: 10.1126/science.182.4109.296.
8
The occurrence of single-stranded DNA in the serum of patients with systemic lupus erythematosus and other diseases.系统性红斑狼疮及其他疾病患者血清中单链DNA的出现情况。
J Clin Invest. 1973 Jan;52(1):198-204. doi: 10.1172/JCI107165.
9
Nucleic acid antibodies in African trypanosomiasis: studies in Rhesus monkeys and man.非洲锥虫病中的核酸抗体：恒河猴和人类研究
J Immunol. 1974 Dec;113(6):1921-7.
10
Release of DNA in circulating blood and induction of anti-DNA antibodies after injection of bacterial lipopolysaccharides.注射细菌脂多糖后循环血液中DNA的释放及抗DNA抗体的诱导。
J Exp Med. 1974 Nov 1;140(5):1189-206. doi: 10.1084/jem.140.5.1189.