Sar M, Stumpf W E
Science. 1973 Dec 21;182(4118):1266-8. doi: 10.1126/science.182.4118.1266.
Selective concentration of [(1)H]progesterone or its metabolites is observed in nuclei of neurons in certain hypothalamic regions of the guinea pig 15 minutes after injection of [1,2,6,7-(3)H]progesterone, by use of dry-mount autoradiography. Highest concentrations of progestin target neurons exist in the nucleus arcuatus, the nucleus preopticus periventricularis, and the nucleus preopticus suprachiasmaticus. Previous administration of unlabeled progesterone inhibits the nuclear concentration of radioactivity, but cortisol has no effect. Estradiol priming enhances the nuclear uptake of radioactivity. The results demonstrate the existence of progestin target sites in the hypothalamus and suggest a direct action of progestin on certain hypothalamic structures.
通过干板放射自显影法观察到，豚鼠在注射[1,2,6,7-³H]孕酮15分钟后，特定下丘脑区域的神经元核中存在[(1)H]孕酮或其代谢物的选择性浓集。孕激素靶神经元的最高浓度存在于弓状核、室周视前核和视交叉上视前核。预先给予未标记的孕酮可抑制放射性核素的核浓集，但皮质醇无此作用。雌二醇预处理可增强放射性核素的核摄取。结果表明下丘脑中存在孕激素靶位点，并提示孕激素对某些下丘脑结构有直接作用。
