Colvin J R, Sowden L C, van den Berg L
Can J Microbiol. 1979 Jul;25(7):826-32. doi: 10.1139/m79-122.
The ultrastructure of the cells of the major component of an enriched culture of a presumed methanogen which utilized acetic acid was studied by transmission and scanning electron microscopy. The filaments were composed of Gram-positive, rod-shaped cells, 1--2 micrometer in length and about 0.5 micrometer in breadth, attached end to end. Septa between cells were complex, with a central, electron-dense sheet which had a spherical enlargement in the center separating the cell walls. The cells walls themselves were of variable thickness with a light, fluffy, thin portion on the outside and a denser, thicker portion within. They contain a series of rings stacked side by side which are composed of material that stains strongly and positively with phosphotungstate ion. The cytoplasmic membrane of these cells had an outer leaflet which stains more densely with uranium and lead ions than the inner leaflet. There were no recognizable organelles in the cytoplasm other than ribosomes. It is shown in these observations that the presumed methanogen may likely be a new species.
利用透射电子显微镜和扫描电子显微镜对一种假定利用乙酸的产甲烷菌富集培养物主要成分的细胞超微结构进行了研究。这些丝状体由革兰氏阳性、杆状细胞组成，细胞长1 - 2微米，宽约0.5微米，首尾相连。细胞间的隔膜很复杂，有一个中央电子致密片层，其中心有一个球形膨大，将细胞壁隔开。细胞壁本身厚度不一，外侧有一层薄而轻、蓬松的部分，内侧有一层更致密、更厚的部分。它们含有一系列并排堆叠的环，这些环由与磷钨酸离子强烈正染色的物质组成。这些细胞的细胞质膜外小叶比内小叶更密集地被铀和铅离子染色。除核糖体外，细胞质中没有可识别的细胞器。这些观察结果表明，这种假定的产甲烷菌很可能是一个新物种。
