Yoshida S, Yamada M, Masaki S, Saneyoshi M
Cancer Res. 1979 Oct;39(10):3955-8.
Chemically synthesized beta-2'-deoxy-6-thioguanosine 5'-triphosphate, a putative active form of beta-2'-deoxy-6-thioguanosine, was used efficiently as a substrate for DNA synthesis catalyzed by DNA polymerase alpha from calf thymus. The deoxythioguanylate was incorporated into DNA by replacing deoxyguanylate and supported the further elongation of DNA chains on activated calf thymus DNA. In contrast, DNA polymerase beta used beta-2'-deoxy-6-thioguanosine 5'-triphosphate at a much lower rate. The reaction product of DNA polymerase alpha, i.e., 6-thioguanine-containing DNA, adsorbed specifically to the organomercurial agarose column, and showed a peak of UV absorption at 342 nm, which is characteristic of thioguanine.
化学合成的β-2'-脱氧-6-硫代鸟苷5'-三磷酸，一种推测的β-2'-脱氧-6-硫代鸟苷活性形式，被有效地用作小牛胸腺DNA聚合酶α催化的DNA合成的底物。脱氧硫代鸟苷酸通过取代脱氧鸟苷酸掺入DNA中，并支持在活化的小牛胸腺DNA上进一步延长DNA链。相比之下，DNA聚合酶β使用β-2'-脱氧-6-硫代鸟苷5'-三磷酸的速率要低得多。DNA聚合酶α的反应产物，即含6-硫代鸟嘌呤的DNA，特异性吸附到有机汞琼脂糖柱上，并在342nm处显示紫外线吸收峰，这是硫代鸟嘌呤的特征。
