免疫反应的遗传控制。以水溶液形式给予的抗原剂量对于确定哪种小鼠品系对聚（L-酪氨酸，L-谷氨酸）-聚（L-脯氨酸）-聚（L-赖氨酸）是高反应者至关重要。

Genetic control of immune response. The dose of antigen given in aqueous solution is critical in determining which mouse strain is high responder to poly(LTyr, LGlu)-poly(LPro)--poly(LLys).

作者信息

Jormalainen S, Mozes E, Sela M

出版信息

J Exp Med. 1975 May 1;141(5):1057-72. doi: 10.1084/jem.141.5.1057.

DOI:10.1084/jem.141.5.1057

PMID:47893

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2189793/

Abstract

Antibody response to different doses of (T,G)-Pro--L, given in aqueous solution, was investigated in the high responder SJL and low responder DBA/1 strains by measuring hemolytic plaque-forming cells (PFC) in the spleens as well as hemagglutination titers in the sera. The gene responsible for the difference between the two strains in the response to this antigen, given in complete Freund's adjuvant, has been previously denoted Ir-3. This gene is not linked to the major histocompatibility locus. In the response to the optimal dose (1 mug) of antigen, no difference could be shown between the strains. The peak of the response and the numbers of direct and indirect PFC were similar in both strains in the primary and secondary response. After injection of higher doses (10-100 mug) of antigen, both the direct and indirect PFC responses were lower in the low responder than in the high responder strain. Moreover, the peak of the response occurred earlier in the high responder strain in the primary response to the 10 mu dose of antigen. After administration of a suboptimal dose (0.02 mug) of antigen, the low responder strain produced in the primary response 4-20 times more indirect plaques than the high responder strain. Also the number of direct plaques was higher in the low responder than in the high responder strain. The serum antibody responses to the optimal and higher doses of antigen were parallel to the PFC responses. From inhibition of PFC with free antigen, it was concluded that a similar proportion of cells was producing high and low affinity antibodies to (T,G)-Pro--L in both strains. High and low zone tolerance could be induced in the two strains with (T,G)-Pro--L, but no difference could be shown between the strains. It is suggested that the Ir-3 gene plays a role in the regulation of the balance stimulation and suppression according to the dose of antigen given.

摘要

通过测量脾脏中的溶血空斑形成细胞（PFC）以及血清中的血凝滴度，研究了高反应性SJL品系和低反应性DBA/1品系对不同剂量（T，G）-Pro-L水溶液的抗体反应。先前已将负责这两个品系对完全弗氏佐剂中该抗原反应差异的基因命名为Ir-3。该基因与主要组织相容性位点不连锁。在对抗原最佳剂量（1微克）的反应中，两个品系之间未显示出差异。在初次和二次反应中，两个品系的反应峰值以及直接和间接PFC的数量相似。注射更高剂量（10 - 100微克）的抗原后，低反应性品系的直接和间接PFC反应均低于高反应性品系。此外，在对10微克剂量抗原的初次反应中，高反应性品系的反应峰值出现得更早。给予次最佳剂量（0.02微克）的抗原后，低反应性品系在初次反应中产生的间接空斑比高反应性品系多4 - 20倍。低反应性品系的直接空斑数量也高于高反应性品系。血清抗体对最佳和更高剂量抗原的反应与PFC反应平行。通过用游离抗原抑制PFC得出结论，两个品系中产生高亲和力和低亲和力抗体的细胞比例相似。（T，G）-Pro-L可在两个品系中诱导高区和低区耐受性，但两个品系之间未显示出差异。提示Ir-3基因根据给予的抗原剂量在刺激和抑制平衡的调节中起作用。