棕色固氮菌转化的最佳条件。
Optimal conditions for transformation of Azotobacter vinelandii.
作者信息
Page W J, von Tigerstrom M
出版信息
J Bacteriol. 1979 Sep;139(3):1058-61. doi: 10.1128/jb.139.3.1058-1061.1979.
Abstract
Optimal transformation of Azotobacter vinelandii OP required a 20-min incubation of the competent cells with deoxyribonucleic acid at 30 degrees C in buffer (pH 6.0 to 8.0) containing 8 mM magnesium sulfate. Nitrogen-fixing transformants of nitrogen fixation-deficient recipients could be plated immediately on selective medium, but transformants acquiring rifampin and streptomycin resistance required preincubation in nonselective medium. The three phenotypes achieved an approximately equal and stable frequency after 17 h (six generations) of growth in nonselective medium.
摘要
棕色固氮菌OP的最佳转化需要感受态细胞在含有8 mM硫酸镁、pH值为6.0至8.0的缓冲液中于30℃下与脱氧核糖核酸孵育20分钟。固氮缺陷型受体的固氮转化体可立即接种在选择性培养基上，但获得利福平和链霉素抗性的转化体需要在非选择性培养基中预培养。这三种表型在非选择性培养基中生长17小时（六代）后达到大致相等且稳定的频率。
相似文献
1
Optimal conditions for transformation of Azotobacter vinelandii.棕色固氮菌转化的最佳条件。
J Bacteriol. 1979 Sep;139(3):1058-61. doi: 10.1128/jb.139.3.1058-1061.1979.
2
Physiological factors affecting transformation of Azotobacter vinelandii.影响棕色固氮菌转化的生理因素。
J Bacteriol. 1976 Mar;125(3):1080-7. doi: 10.1128/jb.125.3.1080-1087.1976.
3
Transformation of Azotobacter vinelandii strains unable to fix nitrogen with Rhizobium spp. DNA.用根瘤菌属DNA对不能固氮的棕色固氮菌菌株进行转化。
Can J Microbiol. 1978 Mar;24(3):209-14. doi: 10.1139/m78-038.
4
Transformation of Azotobacter vinelandii OP with a broad host range plasmid containing a cloned chromosomal nif-DNA marker.用含有克隆的染色体固氮基因DNA标记的广宿主范围质粒转化棕色固氮菌OP。
Plasmid. 1988 May;19(3):242-50. doi: 10.1016/0147-619x(88)90042-x.
5
Plasmid transformation of Azotobacter vinelandii OP.棕色固氮菌OP的质粒转化
J Gen Microbiol. 1987 Aug;133(8):2059-72. doi: 10.1099/00221287-133-8-2059.
6
UV-repair and mutagenesis in Azotobacter vinelandii. II. Repair and mutagenesis.棕色固氮菌中的紫外线修复与诱变。II. 修复与诱变。
Zentralbl Mikrobiol. 1985;140(3):255-8.
7
Isolation of ntrA-like mutants of Azotobacter vinelandii.棕色固氮菌ntrA样突变体的分离
J Bacteriol. 1986 May;166(2):541-4. doi: 10.1128/jb.166.2.541-544.1986.
8
Nitrogen fixation system of tungsten-resistant mutants of Azotobacter vinelandii.维涅兰德固氮菌耐钨突变体的固氮系统
J Bacteriol. 1982 Oct;152(1):72-80. doi: 10.1128/jb.152.1.72-80.1982.
9
Control of transformation competence in Azotobacter vinelandii by nitrogen catabolite derepression.通过氮代谢物去阻遏控制维涅兰德固氮菌的转化能力。
J Bacteriol. 1976 Mar;125(3):1088-95. doi: 10.1128/jb.125.3.1088-1095.1976.
10
Transformation of Azotobacter vinelandii with plasmid DNA.用质粒DNA转化棕色固氮菌。
J Bacteriol. 1985 Apr;162(1):276-9. doi: 10.1128/jb.162.1.276-279.1985.
引用本文的文献
1
Competitive fitness and stability of ammonium-excreting Azotobacter vinelandii strains in the soil.在土壤中具有氨排泄能力的固氮菌（Azotobacter vinelandii）菌株的竞争适应性和稳定性。
Appl Microbiol Biotechnol. 2024 Jun 18;108(1):378. doi: 10.1007/s00253-024-13231-1.
2
Role of Serine Coordination in the Structural and Functional Protection of the Nitrogenase P-Cluster.丝氨酸配位在氮酶 P 簇结构和功能保护中的作用。
J Am Chem Soc. 2022 Dec 7;144(48):22101-22112. doi: 10.1021/jacs.2c09480. Epub 2022 Nov 29.
3
A critical role of an oxygen-responsive gene for aerobic nitrogenase activity in Azotobacter vinelandii and its application to Escherichia coli.氧响应基因在好气固氮菌和其在大肠杆菌中的应用中对有氧氮酶活性的关键作用。
Sci Rep. 2022 Mar 9;12(1):4182. doi: 10.1038/s41598-022-08007-4.
4
Genetic Determinants of Ammonium Excretion in Mutants of Azotobacter vinelandii.固氮菌属突变体铵排泄的遗传决定因素。
Appl Environ Microbiol. 2022 Mar 22;88(6):e0187621. doi: 10.1128/AEM.01876-21. Epub 2022 Feb 9.
5
Redox-Dependent Metastability of the Nitrogenase P-Cluster.氮酶 P 簇的氧化还原依赖的亚稳性。
J Am Chem Soc. 2019 Jun 26;141(25):10091-10098. doi: 10.1021/jacs.9b04555. Epub 2019 Jun 13.
6
Azotobacter vinelandii Nitrogenase Activity, Hydrogen Production, and Response to Oxygen Exposure.威兰固氮菌氮酶活性、氢气生成和对氧气暴露的响应。
Appl Environ Microbiol. 2018 Aug 1;84(16). doi: 10.1128/AEM.01208-18. Print 2018 Aug 15.
7
The Electron Bifurcating FixABCX Protein Complex from Azotobacter vinelandii: Generation of Low-Potential Reducing Equivalents for Nitrogenase Catalysis.来自棕色固氮菌的电子分叉FixABCX蛋白复合物：为固氮酶催化产生低电位还原当量
Biochemistry. 2017 Aug 15;56(32):4177-4190. doi: 10.1021/acs.biochem.7b00389. Epub 2017 Aug 3.
8
Protons and pleomorphs: aerobic hydrogen production in Azotobacters.质子与多形体：固氮菌中的好氧产氢
World J Microbiol Biotechnol. 2016 Feb;32(2):29. doi: 10.1007/s11274-015-1980-5. Epub 2016 Jan 9.
9
Kinetics of Nif gene expression in a nitrogen-fixing bacterium.固氮菌中 Nif 基因表达的动力学。
J Bacteriol. 2014 Feb;196(3):595-603. doi: 10.1128/JB.00942-13. Epub 2013 Nov 15.
10
NifEN-B complex of Azotobacter vinelandii is fully functional in nitrogenase FeMo cofactor assembly.固氮菌中非血红素铁蛋白 NifEN-B 复合物在固氮酶 FeMo 辅因子组装中完全功能化。
Proc Natl Acad Sci U S A. 2011 May 24;108(21):8623-7. doi: 10.1073/pnas.1102773108. Epub 2011 May 5.
本文引用的文献
1
BIOSYNTHETIC LATENCY IN EARLY STAGES OF DEOXYRIBONUCLEIC ACIDTRANSFORMATION IN BACILLUS SUBTILIS.枯草芽孢杆菌中脱氧核糖核酸转化早期阶段的生物合成潜伏期
J Bacteriol. 1963 Oct;86(4):785-96. doi: 10.1128/jb.86.4.785-796.1963.
2
INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM.脱氧核糖核酸在枯草芽孢杆菌转化系统中的掺入
J Bacteriol. 1963 Sep;86(3):392-400. doi: 10.1128/jb.86.3.392-400.1963.
3
Fate of transforming deoxyribonucleate following fixation by transformable bacteria.可转化细菌固定后转化脱氧核糖核酸的命运
Nature. 1960 Sep 17;187:1002-6. doi: 10.1038/1871002a0.
4
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
5
Hydrogen ion buffers for biological research.用于生物学研究的氢离子缓冲剂。
Biochemistry. 1966 Feb;5(2):467-77. doi: 10.1021/bi00866a011.
6
Factors affecting transformation of Micrococcus lysodeikticus.影响溶壁微球菌转化的因素。
J Bacteriol. 1969 Jun;98(3):1397-9. doi: 10.1128/jb.98.3.1397-1399.1969.
7
Loss of activity of transforming deoxyribonucleic acid after uptake by Haemophilus influenzae.流感嗜血杆菌摄取后转化脱氧核糖核酸活性的丧失。
J Bacteriol. 1965 Oct;90(4):873-83. doi: 10.1128/jb.90.4.873-883.1965.
8
The nature of the transformation process in Escherichia coli K12.大肠杆菌K12中转化过程的本质。
Mol Gen Genet. 1973 Jul 31;124(1):1-10. doi: 10.1007/BF00267159.
9
Factors affecting competence for transformation in Staphylococcus aureus.影响金黄色葡萄球菌转化能力的因素。
J Bacteriol. 1974 Apr;118(1):155-64. doi: 10.1128/jb.118.1.155-164.1974.
10
Further observations on genetic transformation in pseudomonas.关于假单胞菌遗传转化的进一步观察
J Gen Microbiol. 1974 Aug;83(2):251-9. doi: 10.1099/00221287-83-2-251.
Zotero 插件