Shurin S B, Socransky S S, Sweeney E, Stossel T P
N Engl J Med. 1979 Oct 18;301(16):849-54. doi: 10.1056/NEJM197910183011601.
We recovered capnocytophaga, a gram-negative anaerobe implicated in the pathogenesis of periodontal disease, from two patients with a history of dental infections. Neutrophils from both patients failed to acquire the asymmetric shape characteristic of normal neutrophils. Fluorescein staining of the patients' living neutrophils remained diffuse and patchy instead of showing the normal pattern in which the fluorescence is swept into the rear of the cell. The locomotion of one patient's neutrophils in vitro was less than 50 per cent of that of normal neutrophils, and migration of this patient's neutrophils into dermal abrasions was reduced, although phagocytosis and nitroblue tetrazolium reduction were normal. All abnormalities of neutrophil morphology and function disappeared after eradication of the capnocytophaga infections. Sonicates and culture medium of capnocytophaga contained a dialyzable substance that caused normal neutrophils to behave like neutrophils obtained from the infected patients.
我们从两名有牙齿感染病史的患者身上分离出了二氧化碳嗜纤维菌，这是一种与牙周病发病机制有关的革兰氏阴性厌氧菌。两名患者的中性粒细胞均未呈现正常中性粒细胞特有的不对称形态。对患者活中性粒细胞进行荧光素染色后，荧光仍呈弥漫性和斑片状，而未呈现出正常的荧光扫入细胞尾部的模式。一名患者的中性粒细胞在体外的运动能力不到正常中性粒细胞的50%，并且该患者的中性粒细胞向皮肤擦伤处的迁移减少，不过吞噬作用和硝基蓝四氮唑还原反应正常。在根除二氧化碳嗜纤维菌感染后，中性粒细胞形态和功能的所有异常均消失。二氧化碳嗜纤维菌的超声裂解物和培养基中含有一种可透析物质，该物质可使正常中性粒细胞表现得如同从感染患者身上获取的中性粒细胞一样。
