Tuazon C U, Perez A A, Elin R J, Sheagren J N
Arch Intern Med. 1977 Jan;137(1):55-6.
Cerebrospinal fluid (CSF) and joint fluid from 81 patients were studied for the presence of endotoxin using the limulus assay. All fluids that grew Gram-negative organisms had a positive limulus test. However, one sample of CSF and one of joint fluid, both very purulent, that contained Gram-positive organisms also gave positive limulus assays. All culturally negative fluids had a negative limulus assay. Thus, a positive limulus assay with CSF or joint fluid is indicative of bacterial infection probably caused by Gram-negative organisms and can be an extremely useful diagnostic adjunct.
使用鲎试剂法对81例患者的脑脊液（CSF）和关节液进行内毒素检测。所有培养出革兰氏阴性菌的液体鲎试剂检测均呈阳性。然而，一份脑脊液样本和一份关节液样本，二者均为脓性且含有革兰氏阳性菌，鲎试剂检测也呈阳性。所有培养阴性的液体鲎试剂检测均为阴性。因此，脑脊液或关节液鲎试剂检测呈阳性表明可能由革兰氏阴性菌引起的细菌感染，可作为一项极为有用的辅助诊断手段。
