Naylor S L, Townsend J K, Klebe R J
Somatic Cell Genet. 1979 Mar;5(2):271-7. doi: 10.1007/BF01539166.
In a previous study, several cultured cell lines were detected which are naturally occurring auxotrophs. In this investigation, the enzyme deficienceis involved are described. It is demonstrated that the Chinese hamster cell lines CHO(K1), YH21, RJK-36, and CHW-1102 are deficient in cystathionase and argininosuccinate synthetase. In addition, CHO (K1) and CHW-1102 were found to lack argininosuccinate lyase. CHW-1102 cells were also found to be unable to proliferate in medium containing branched-chain alpha-keto acids in place of the corresponding L-amino acids since CHW-1102 cells lack branched-chain aminotransferase.
在先前的一项研究中，检测到几种天然存在的营养缺陷型培养细胞系。在本研究中，描述了所涉及的酶缺陷。结果表明，中国仓鼠细胞系CHO(K1)、YH21、RJK - 36和CHW - 1102缺乏胱硫醚酶和精氨琥珀酸合成酶。此外，发现CHO(K1)和CHW - 1102缺乏精氨琥珀酸裂解酶。还发现CHW - 1102细胞在含有支链α-酮酸而非相应L-氨基酸的培养基中无法增殖，因为CHW - 1102细胞缺乏支链氨基转移酶。
