高渗培养基及振荡在菌血症检测中的批判性分析
Critical analysis of hypertonic medium and agitation in detection of bacteremia.
作者信息
Ellner P D, Kiehn T E, Beebe J L, McCarthy L R
出版信息
J Clin Microbiol. 1976 Sep;4(3):216-24. doi: 10.1128/jcm.4.3.216-224.1976.
Abstract
Over 18,000 clinical specimens collected in Vacutainer tubes with sodium polyanethol sulfonate were inoculated into modified Columbia broth (MCB) with and without 10% sucrose. The effects of venting and shaking on recovery were studied. The volume of the blood had a definite effect on the recovery rate. When inoculum size was held constant, recovery of aerobic and facultative organisms was maximal in vented and shaken bottles; the presence of sucrose had no demonstrable effect, recovery of anaerobes was maximal using an unvented bottle incubated under stationary conditions; a significantly greater recovery of facultatives and a marginally greater recovery of anaerobes was obtained with the hypertonic formulation. We conclude that a hypertonic formulation of MCB offers no advantage in the recovery of anaerobes but is of value in the recovery of facultatives and anaerobes. It is recommended that blood cultures be routinely inoculated into isotonic MCB and then vented and shaken for at least 4 hours, and hypertonic MCB incubated without venting or shaking.
摘要
将超过18000份采集于含有聚茴香脑磺酸钠的真空采血管中的临床标本接种到添加和未添加10%蔗糖的改良哥伦比亚肉汤（MCB）中。研究了通气和振荡对回收率的影响。血液体积对回收率有明确影响。当接种量保持恒定时，在通气并振荡的瓶中，需氧菌和兼性菌的回收率最高；蔗糖的存在没有明显影响，在静止条件下培养的未通气瓶中厌氧菌的回收率最高；使用高渗配方时，兼性菌的回收率显著更高，厌氧菌的回收率略高。我们得出结论，MCB的高渗配方在厌氧菌的回收率方面没有优势，但在兼性菌和厌氧菌的回收率方面有价值。建议将血培养常规接种到等渗MCB中，然后通气并振荡至少4小时，高渗MCB则在不通气或不振荡的情况下培养。
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