Catty D, Chambers L, Lowe J A
Immunology. 1974 Feb;26(2):331-43.
Rabbits born as locus genotypic b4, 6 heterozygotes, but exposed or neonatally to anti-b6 antibody, show chronic phenotypic suppression of the synthesis of the b6 allele in serum immunoglobulin. The suppression has been quantified over an extended period in relation to the synthesis of the alternative locus allele (b4), locus determinants, and total IgG. locus suppression does not alter the synthesis or proportions of locus (a1 and a3) determinants. The abrogation of suppression which can be effected neonatally by injection of immunoglobulin appears to be an antibody-neutralizing event since it requires specifically b6 determinants and cannot be achieved with non-b6 (5,9) complexes formed . Neutralization results in the rapid establishment of a b6 immunoglobulin-synthesizing cell population. Two assays, the mixed rosette and lymphocyte transformation tests with anti-allotype sera, have been used to examine the influence of suppression and suppression-neutralization on the distribution of allotypic determinants on immunoglobulin receptor bearing lymphocytes in the peripheral circulation and lymphoid tissues. In b6-suppressed rabbits there is an absence of b6-carrying lymphocytes in all the lymphoid tissues examined. In suppression-neutralized rabbits, however, such cells are present in numbers which correlate reasonably well with the proportion of b6 in the serum immunoglobulin. In rabbits in which no b6 was detectable at any time in the serum to 100 days of age, intensive immunization with b6 did not result in production of antibody to the b6 determinant. These findings are discussed in relation to proposals of the mechanism of induction and maintenance of chronic suppression in the rabbit.
出生时为基因座基因型b4、6杂合子，但在新生期接触抗b6抗体的兔子，血清免疫球蛋白中b6等位基因的合成表现出慢性表型抑制。相对于替代基因座等位基因（b4）的合成、基因座决定簇和总IgG，这种抑制在较长时间内已被量化。基因座抑制不会改变基因座（a1和a3）决定簇的合成或比例。新生期通过注射免疫球蛋白可实现的抑制解除似乎是一个抗体中和事件，因为它需要特异性的b6决定簇，且不能通过形成的非b6（5,9）复合物来实现。中和导致b6免疫球蛋白合成细胞群体迅速建立。已使用两种检测方法，即混合玫瑰花结试验和用抗同种异型血清进行的淋巴细胞转化试验，来研究抑制和抑制解除对外周循环和淋巴组织中携带免疫球蛋白受体的淋巴细胞上同种异型决定簇分布的影响。在b6抑制的兔子中，在所检查的所有淋巴组织中均不存在携带b6的淋巴细胞。然而，在抑制解除的兔子中，此类细胞的数量与血清免疫球蛋白中b6的比例有合理的相关性。在100日龄前血清中任何时候都检测不到b6的兔子中，用b6进行强化免疫不会导致产生针对b6决定簇的抗体。将结合兔子慢性抑制的诱导和维持机制的相关提议来讨论这些发现。
