组蛋白可刺激鼠白血病病毒介导的多聚核糖核苷酸指导的多聚脱氧核糖核苷酸合成。

Histones stimulate polyribonucleotide-directed polydeoxyribonucleotide synthesis by murine leukemia virus.

作者信息

Manly K F

出版信息

J Virol. 1974 Feb;13(2):305-11. doi: 10.1128/JVI.13.2.305-311.1974.

DOI:10.1128/JVI.13.2.305-311.1974

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC355298/

Abstract

The rate of homoribopolymer-directed DNA synthesis by detergent-disrupted Moloney murine leukemia virus can be stimulated or inhibited by histone, depending on the ratio of histone to template. Of the fractions which can be separated from the whole histone, f1 causes both the greatest stimulation and the greatest inhibition. The effect of histone f1 is qualitatively similar whether the template is polyadenylate (poly A), polycytidylate, or polyuridylate, but the stimulation is greatest with poly A. The pattern of stimulation and inhibition differs, however, for a different polymerase; the DNA polymerase of Micrococcus luteus is inhibited by histone concentrations which stimulate the viral enzyme and stimulated by concentrations which inhibit the viral enzyme. For the viral enzyme, the optimum histone concentration is unaffected by changes in the virus or primer concentration; but it varies in proportion to the template concentration, suggesting that histone acts by combining stoichiometrically with the template. These data raise the possibility that a histone-like protein may participate in the synthesis of the provirus of RNA tumor viruses.

摘要

去污剂裂解的莫洛尼氏鼠白血病病毒进行同聚核糖核苷酸指导的DNA合成的速率，可被组蛋白刺激或抑制，这取决于组蛋白与模板的比例。在可从全组蛋白中分离出的组分中，f1组分引起的刺激和抑制作用最大。无论模板是聚腺苷酸（poly A）、聚胞苷酸还是聚尿苷酸，组蛋白f1的作用在性质上都是相似的，但对poly A的刺激作用最大。然而对于另一种不同的聚合酶，刺激和抑制模式有所不同；藤黄微球菌的DNA聚合酶会被刺激病毒酶的组蛋白浓度所抑制，而被抑制病毒酶的组蛋白浓度所刺激。对于病毒酶而言，最佳组蛋白浓度不受病毒或引物浓度变化的影响；但它与模板浓度成比例变化，这表明组蛋白通过与模板化学计量结合而起作用。这些数据增加了一种可能性，即一种类似组蛋白的蛋白质可能参与RNA肿瘤病毒前病毒的合成。

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