Orstavik D, Kraus F W, Henshaw L C
Infect Immun. 1974 May;9(5):794-800. doi: 10.1128/iai.9.5.794-800.1974.
The ability of Streptococcus strains to adhere to the tooth surface in vitro was investigated. Polished enamel slabs, with and without acquired pellicles, were incubated with buffer suspensions of oral streptococci, and attached bacteria were counted under a microscope using incident light. Low numbers of bacteria adhered to uncoated enamel; the presence of an acquired pellicle significantly enhanced the attachment of all strains tested. The adherence of Streptococcus sanguis was significantly greater than that of Streptococcus salivarius, and both of these strains adhered in greater numbers than did Streptococcus mutans. When bacteria were suspended in whole saliva, the adherence of S. salivarius and S. mutans was inhibited, whereas the adherence of S. sanguis was enhanced in some experiments and inhibited in others. The adherence of S. sanguis and S. salivarius was consistently inhibited by parotid fluid; this inhibitory effect persisted after thorough washing and resonication of the bacterial cells. Incubation in oral fluids was associated with the attachment of bacterial clumps to the pellicle, and parallel investigation revealed agglutination of S. sanguis and S. salivarius by whole saliva and, in particular, parotid fluid. The results are discussed in terms of surface microecology, and are related to the development of dental plaque.
研究了链球菌菌株在体外黏附于牙齿表面的能力。将有或没有获得性薄膜的抛光牙釉质片与口腔链球菌的缓冲液悬浮液一起孵育，然后在显微镜下利用入射光对附着的细菌进行计数。少量细菌黏附于未包被的牙釉质；获得性薄膜的存在显著增强了所有测试菌株的黏附。血链球菌的黏附明显大于唾液链球菌，且这两种菌株的黏附数量均多于变形链球菌。当细菌悬浮于全唾液中时，唾液链球菌和变形链球菌的黏附受到抑制，而在某些实验中血链球菌的黏附增强，在另一些实验中则受到抑制。腮腺液始终抑制血链球菌和唾液链球菌的黏附；在对细菌细胞进行彻底洗涤和再超声处理后，这种抑制作用仍然存在。在口腔液体中孵育与细菌团块黏附于薄膜有关，同时的研究表明全唾液尤其是腮腺液可使血链球菌和唾液链球菌发生凝集。从表面微生态学角度对结果进行了讨论，并与牙菌斑的形成相关。
