胰蛋白酶处理的人O型红细胞作为日本脑炎病毒的一种替代血凝剂。
Trypsinized human group O erythrocytes as an alternative hemagglutinating agent for Japanese encephalitis virus.
作者信息
Shortridge K F, Hu L Y
出版信息
Appl Microbiol. 1974 Apr;27(4):653-6. doi: 10.1128/am.27.4.653-656.1974.
Trypsinized human group O erythrocytes were found to be a suitable alternative to gander cells in hemagglutination (HA) and hemagglutination inhibition (HAI) tests for Japanese encephalitis (JE) virus. In the HAI test, no cross-reactions against JE virus were observed with immune sera containing antibody to taxonomically related or unrelated viruses, with mouse brain antigen, or with nonantibody serum inhibitors; specific antibody rise could be detected in an immunized rabbit. Gander and trypsinized human group O cells gave comparable titers in the HAI test, but the latter were preferable since (i) they required less challenging HA antigen, being more sensitive to agglutination by JE virus, and (ii) all human and some animal sera investigated were devoid of natural agglutinins for these cells, thereby eliminating or reducing the need for prior adsorption with packed cells.
在日本脑炎（JE）病毒的血凝（HA）和血凝抑制（HAI）试验中，发现胰蛋白酶处理的人O型红细胞是替代雄鹅细胞的合适选择。在HAI试验中，针对JE病毒，含有针对分类学上相关或不相关病毒的抗体的免疫血清、小鼠脑抗原或非抗体血清抑制剂均未观察到交叉反应；在免疫兔中可检测到特异性抗体升高。在HAI试验中，雄鹅细胞和胰蛋白酶处理的人O型细胞给出了相当的滴度，但后者更可取，因为（i）它们所需的挑战性HA抗原较少，对JE病毒的凝集更敏感，并且（ii）所研究的所有人和一些动物血清都不含针对这些细胞的天然凝集素，从而消除或减少了用压积细胞预先吸附的需要。
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