来自静止小麦胚的多种脱氧核糖核酸聚合酶。可溶性细胞质中三种酶和纯化线粒体中一种酶的纯化及特性分析。
Multiple deoxyribonucleic acid polymerases from quiescent wheat embryos. Purification and characterization of three enzymes from the soluble cytoplasm and one from purified mitochondria.
作者信息
Castroviejo M, Tharaud D, Tarrago-Litvak L, Litvak S
出版信息
Biochem J. 1979 Jul 1;181(1):183-91. doi: 10.1042/bj1810183.
Abstract
Three DNA polymerases (A, B and C) have been purified from the soluble cytoplasm of ungerminated embryos. Mainly on the basis of chromatographic, template-specificity and salt-inhibition evidence, we have characterized the three enzymes. Other physico-chemical and enzymic properties are described. From purified mitochondria we have purified a DNA polymerase that behaves like DNA polymerase B on chromatographic and template-specificity criteria. Only highly purified enzyme B from the soluble cytoplasm showed an exonuclease activity able to degrade 3'- or 5'-labelled polydeoxyribonucleotides, as well as a 'proof-reading' capacity.
摘要
已从未萌发胚胎的可溶性细胞质中纯化出三种DNA聚合酶（A、B和C）。主要基于色谱分析、模板特异性和盐抑制证据，我们对这三种酶进行了表征。还描述了其他物理化学和酶学性质。我们从纯化的线粒体中纯化出一种DNA聚合酶，根据色谱分析和模板特异性标准，它的行为与DNA聚合酶B相似。只有从可溶性细胞质中高度纯化的酶B显示出能够降解3'或5'标记的多脱氧核糖核苷酸的核酸外切酶活性，以及“校对”能力。
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