12-α-[³H]-地高辛与完整人红细胞膜结合的特性：与地高辛治疗的相关性。
The characteristics of the binding of 12-alpha-[3H]-digoxin to the membranes of intact human erythrocytes: relevance to digoxin therapy.
作者信息
Ford A R, Aronson J K, Grahame-Smith D G, Rose J A
出版信息
Br J Clin Pharmacol. 1979 Aug;8(2):115-24. doi: 10.1111/j.1365-2125.1979.tb05809.x.
Abstract
1 The characteristics of the binding of 12-alpha-[3H]-digoxin to the membranes of intact human erythrocytes are described. 2 Only one class of binding site can be demonstrated. Binding is time- and temperature-dependent, saturable and slowly reversible; it is inhibited by other cardiac glycosides and by potassium. 3 Pre-incubation with unlabelled digoxin reduces the subsequent binding of 12-alpha-[3H]-digoxin in stoichiometric fashion. 4 The possible application of the measurement of the binding of 12-alpha-[3H]-digoxin to the study of biochemical pharmacological events occurring during digoxin therapy is discussed.
摘要
- 本文描述了12-α-[³H]-地高辛与完整人红细胞膜结合的特性。2. 仅能证明存在一类结合位点。结合具有时间和温度依赖性、可饱和且可逆性缓慢；它受到其他强心苷和钾的抑制。3. 与未标记的地高辛预孵育以化学计量方式降低随后12-α-[³H]-地高辛的结合。4. 讨论了测定12-α-[³H]-地高辛结合在研究地高辛治疗期间发生的生化药理事件中的可能应用。
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The characteristics of the binding of 12-alpha-[3H]-digoxin to the membranes of intact human erythrocytes: relevance to digoxin therapy.12-α-[³H]-地高辛与完整人红细胞膜结合的特性：与地高辛治疗的相关性。
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本文引用的文献
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[Cardiac glycosides as inhibitors of active potassium and sodium transport by erythrocyte membrane].[强心苷作为红细胞膜主动钾和钠转运的抑制剂]
Helv Physiol Pharmacol Acta. 1953;11(4):346-54.
2
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3
The effects of sodium and potassium on ouabain binding by human erythrocytes.钠和钾对人红细胞哇巴因结合的影响。
J Gen Physiol. 1972 Nov;60(5):609-29. doi: 10.1085/jgp.60.5.609.
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Binding of the cardiac glycoside ouabain to intact cells.强心苷哇巴因与完整细胞的结合。
J Physiol. 1972 Jul;224(2):441-62. doi: 10.1113/jphysiol.1972.sp009904.
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Binding of tritiated digoxin to human red cell ghosts.氚标记地高辛与人红细胞影的结合
Nature. 1969 Feb 22;221(5182):776. doi: 10.1038/221776a0.
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Biochem Pharmacol. 1973 Dec 1;22(23):3099-108. doi: 10.1016/0006-2952(73)90196-2.
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The acute changes seen in cardiac glycoside receptor sites, 86rubidium uptake and intracellular sodium concentrations in the erythrocytes of patients during the early phases of digoxin therapy are not found during chronic therapy: pharmacological and therapeutic implications for chronic digoxin therapy.在洋地黄毒苷治疗早期，患者红细胞中强心苷受体位点、86铷摄取及细胞内钠浓度出现的急性变化，在慢性治疗期间并未出现：对慢性洋地黄毒苷治疗的药理学及治疗学意义。
Br J Clin Pharmacol. 1979 Aug;8(2):135-42. doi: 10.1111/j.1365-2125.1979.tb05811.x.
10
Changes in cardiac glycoside receptor sites, 86rubidium uptake and intracellular sodium concentrations in the erythrocytes of patients receiving digoxin during the early phases of treatment of cardiac failure in regular rhythm and of atrial fibrillation.在心力衰竭伴正常节律及心房颤动患者接受地高辛治疗的早期阶段，其红细胞中强心苷受体位点、铷⁸⁶摄取及细胞内钠浓度的变化。
Br J Clin Pharmacol. 1979 Aug;8(2):125-34. doi: 10.1111/j.1365-2125.1979.tb05810.x.
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