Tyler B, Loomis W F, Magasanik B
J Bacteriol. 1967 Dec;94(6):2001-11. doi: 10.1128/jb.94.6.2001-2011.1967.
Severe transient repression of constitutive or induced beta-galactosidase synthesis occurs upon the addition of glucose to cells of Escherichia coli growing on glycerol, succinic acid, or lactic acid. Only mutants particularily well adapted to growth on glucose exhibit this phenomenon when transferred to a glucose-containing medium. No change in ribonucleic acid (RNA) metabolism was observed during transient repression. We could show that transient repression is pleiotropic, affecting all products of the lac operon. It occurs in a mutant insensitive to catabolite repression. It is established much more rapidly than catabolite repression, and is elicited by glucose analogues that are phosphorylated but not further catabolized by the cell. Thus, transient repression is not a consequence of the exclusion of inducer from the cell, does not require catabolism of the added compound, and does not involve a gross change in RNA metabolism. We conclude that transient repression is distinct from catabolite repression.
当向在甘油、琥珀酸或乳酸上生长的大肠杆菌细胞中添加葡萄糖时，组成型或诱导型β-半乳糖苷酶的合成会受到严重的瞬时抑制。只有特别适应在葡萄糖上生长的突变体转移到含葡萄糖的培养基中时才会出现这种现象。在瞬时抑制期间未观察到核糖核酸（RNA）代谢的变化。我们可以证明瞬时抑制是多效性的，影响乳糖操纵子的所有产物。它发生在对分解代谢物阻遏不敏感的突变体中。它比分解代谢物阻遏建立得更快，并且由被细胞磷酸化但不再进一步分解代谢的葡萄糖类似物引发。因此，瞬时抑制不是诱导剂被排除在细胞外的结果，不需要添加化合物的分解代谢，也不涉及RNA代谢的总体变化。我们得出结论，瞬时抑制与分解代谢物阻遏不同。
