通过乳糖信使核糖核酸起始作用和细胞内3',5'-环磷酸腺苷水平测定大肠杆菌中分解代谢物阻遏起始的动力学。
Kinetics of the onset of catabolite repression in Escherichia coli as determined by lac messenger ribonucleic acid initiations and intracellular cyclic adenosine 3',5'-monophosphate levels.
作者信息
Haggerty D M, Schleif R F
出版信息
J Bacteriol. 1975 Sep;123(3):946-53. doi: 10.1128/jb.123.3.946-953.1975.
Abstract
The rates of synthesis of beta-galactosidase (EC 3.2.1.23) and the intracellular levels of cyclic 3',5'-adenosine monophosphate (cAMP) soon after the addition of glucose or glycerol to exponentially growing cultures of Escherichia coli have been determined. Within 10 s of its addition, glucose, but not glycerol, lowered the apparent initiation frequency of lac messenger ribonucleic acid. The glucose-generated reduction in initiations is identified as catabolite repression by its reversibility with cAMP. The intracellular cAMP levels respond virtually identically to glucose and glycerol additions. Thus, no correlation was observed between the rate of messenger ribonucleic acid initiation and the level of cAMP.
摘要
在向指数生长的大肠杆菌培养物中添加葡萄糖或甘油后不久，测定了β-半乳糖苷酶（EC 3.2.1.23）的合成速率以及细胞内环磷酸腺苷（cAMP）的水平。添加葡萄糖后10秒内，而非甘油，降低了乳糖信使核糖核酸的表观起始频率。葡萄糖引起的起始频率降低因其可被cAMP逆转而被确定为分解代谢物阻遏。细胞内cAMP水平对葡萄糖和甘油添加的反应几乎相同。因此，未观察到信使核糖核酸起始速率与cAMP水平之间的相关性。
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Kinetics of the onset of catabolite repression in Escherichia coli as determined by lac messenger ribonucleic acid initiations and intracellular cyclic adenosine 3',5'-monophosphate levels.通过乳糖信使核糖核酸起始作用和细胞内3',5'-环磷酸腺苷水平测定大肠杆菌中分解代谢物阻遏起始的动力学。
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