Lynch D L, Worthy T E, Kresheck G C
Appl Microbiol. 1968 Jan;16(1):13-20. doi: 10.1128/am.16.1.13-20.1968.
A procedure was developed for the separation of pigment fractions in a wild-type Serratia marcescens strain. Separation was achieved by column chromatography and elution with several organic solvents. At least six pigment fractions were obtained from the alumina columns by this technique, whereas only four fractions had been reported previously. Spectral and elemental analyses indicate that, in S. marcescens, prodigiosin is a complex of six fractions, differing in absorption spectra while retaining the general characteristics of the whole pigment.
已开发出一种用于分离野生型粘质沙雷氏菌菌株中色素组分的方法。通过柱色谱法并用几种有机溶剂洗脱来实现分离。用该技术从氧化铝柱中获得了至少六种色素组分,而此前仅报道过四种组分。光谱和元素分析表明,在粘质沙雷氏菌中,灵菌红素是六种组分的复合物,其吸收光谱不同,但保留了整个色素的一般特征。