Anderson J R, Goudie R B, Gray K, Stuart-Smith D A
Clin Exp Immunol. 1968 Feb;3(2):107-17.
Antibodies to adrenocortical cells, occurring in the serum of patients with idiopathic Addison's disease, were investigated by the indirect immunofluorescence technique. With selected human adrenal tissue obtained post mortem, staining was brightest in the innermost cells of the adrenal cortex. Strongly positive sera were observed to react with all thirty specimens of adrenal tissue examined, but lipid-depleted adrenocortical tissue provided the most suitable reagent for detecting weak antibody. Immunofluorescence tests upon twenty positive sera, using a wide range of human tissues, revealed in one serum an antibody, or antibodies, which reacted with all types of cells producing steroid hormones, namely testicular Leydig cells, hilus cells of the ovary and testis, theca interna and corpus luteal cells of the ovary, and placental trophoblast. A second, weaker example of `steroid-cell antibody' was detected among the twenty sera. The significance of steroid-cell antibody is discussed briefly in relation to steroid hormone biosynthesis and gonadal failure in idiopathic Addison's disease.
采用间接免疫荧光技术，对特发性艾迪生病患者血清中出现的肾上腺皮质细胞抗体进行了研究。使用死后获取的选定人类肾上腺组织，肾上腺皮质最内层细胞的染色最为明亮。观察到强阳性血清与所有30份检测的肾上腺组织标本均发生反应，但脂质耗尽的肾上腺皮质组织是检测弱阳性抗体的最合适试剂。使用多种人类组织对20份阳性血清进行免疫荧光检测，在一份血清中发现一种或多种抗体，它们与所有产生类固醇激素的细胞发生反应，即睾丸间质细胞、卵巢和睾丸的门细胞、卵巢的卵泡内膜细胞和黄体细胞以及胎盘滋养层细胞。在这20份血清中检测到第二个较弱的“类固醇细胞抗体”实例。结合特发性艾迪生病中类固醇激素生物合成和性腺功能衰竭，简要讨论了类固醇细胞抗体的意义。
