Donch J, Greenberg J
J Bacteriol. 1968 May;95(5):1555-9. doi: 10.1128/jb.95.5.1555-1559.1968.
The ultraviolet sensitivity gene of Escherichia coli B was introduced into a K-12 recipient by transduction with phage P1. The uvs gene of E. coli B is cotransducible with the proC locus of K-12, is closely linked to tsx, is not linked to lacZ, and only rarely to purE. The transductants are mucoid, filamentous on irradiation, and show plating-medium response. The order of markers is lacZ proC tsx uvs purE.
通过用噬菌体P1转导,将大肠杆菌B的紫外线敏感性基因导入K - 12受体菌。大肠杆菌B的uvs基因与K - 12的proC位点共转导,与tsx紧密连锁,与lacZ不连锁,与purE仅偶尔连锁。转导子呈黏液状,照射后呈丝状,并表现出平板培养基反应。标记顺序为lacZ proC tsx uvs purE。