从土壤和水中分离不动杆菌。
Isolation of Acinetobacter from soil and water.
作者信息
Baumann P
出版信息
J Bacteriol. 1968 Jul;96(1):39-42. doi: 10.1128/jb.96.1.39-42.1968.
Abstract
An enrichment culture procedure for isolating members of the genus Acinetobacter from soil and water is described. It involves the use of vigorously aerated mineral media at relative low pH, supplemented with acetate or other suitable carbon source and nitrate as nitrogen source. With this method, virtually all samples of soil and water yielded representatives of this genus. Semiquantitative comparisons of the numbers of Acinetobacter and of all bacteria capable of aerobic growth in a complex medium revealed that Acinetobacter constituted no less than 0.001% of the total heterotrophic aerobic population in soil and water and was one of the predominant organisms in some water samples.
摘要
本文描述了一种从土壤和水中分离不动杆菌属成员的富集培养程序。该程序涉及在相对较低pH值的矿质培养基中大力曝气，并添加乙酸盐或其他合适的碳源以及硝酸盐作为氮源。使用这种方法，几乎所有土壤和水样都产生了该属的代表菌株。在复杂培养基中对不动杆菌和所有能够有氧生长的细菌数量进行的半定量比较表明，不动杆菌在土壤和水中的总异养需氧菌种群中所占比例不少于0.001%，并且是一些水样中的主要微生物之一。
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