血浆和大脑中的短链脂肪酸：气相色谱法定量测定

Short chain fatty acids in plasma and brain: quantitative determination by gas chromatography.

作者信息

Bachmann C, Colombo J P, Berüter J

出版信息

Clin Chim Acta. 1979 Mar 1;92(2):153-9. doi: 10.1016/0009-8981(79)90109-8.

Abstract

A reliable and practicable method for the determination of short chain fatty acids in plasma and brain tissue is presented. The sample preparation by partition chromatography on silicic acid allows subsequently a quantitation of short chain fatty acids without interference by methylmalonic acid, or other more polar compounds. With the gas-chromatographic system 2-methylbutyrate is separated from isovalerate. Reference values are given for plasma. The system is also useful in combination with mass spectrometry.

摘要

本文提出了一种可靠且实用的测定血浆和脑组织中短链脂肪酸的方法。通过在硅酸上进行分配色谱法进行样品制备，随后可以对短链脂肪酸进行定量，而不受甲基丙二酸或其他极性更强的化合物的干扰。使用气相色谱系统可将2-甲基丁酸与异戊酸分离。文中给出了血浆的参考值。该系统与质谱联用也很有用。