Chollet P, Chassagne J, Philippe P, Vuillaume C, Maublant J, Gauvin H, Rey M, Plagne R
Clin Exp Immunol. 1979 Jul;37(1):152-61.
Few data are available on the blood lymphocyte response to revaccination in man. The anamnestic response to tetanus toxoid challenge was evaluated by a variety of techniques during the first week after revaccination. Out of twenty subjects used, eight were evaluated before and 5 days after the injections (days 1--8). Analytical cell electrophoresis showed important variation in the B and two T lymphocyte populations. The B cell percentages, assessed by EAC-rosettes and electrophoretic mobility, were found to decrease by days 2 and 3, and return to former levels by day 8, when a rise in specific antibodies was detected. A similar response was found in the T1 population generally considered to be composed of low affinity E-rosette-forming cells. Conversely, a significant increase (50--100%) in circulating T2 lymphocytes (active rosette-forming cells) was found by days 2 and 3, followed by a rapid decrease of these 'differenciated' cells. The increase in the T2 lymphocytes appeared earlier in skin test positive subjects. These changes were correlated with E-rosettes, mitogen stimulation, peripheral leucocyte migration inhibition and transformation in the presence of the antigen. EA-IgG rosettes and ADCC varied similarly. These results may indicate a significant non-specific cell mobilization following revaccination.
关于人体再次接种疫苗后血液淋巴细胞反应的数据很少。在再次接种疫苗后的第一周内，通过多种技术评估了对破伤风类毒素激发的回忆反应。在使用的20名受试者中，8名在注射前和注射后5天（第1 - 8天）进行了评估。分析性细胞电泳显示B淋巴细胞群和两个T淋巴细胞群有重要变化。通过EAC玫瑰花结和电泳迁移率评估的B细胞百分比在第2天和第3天下降，并在第8天恢复到先前水平，此时检测到特异性抗体升高。在通常被认为由低亲和力E玫瑰花结形成细胞组成的T1群体中也发现了类似的反应。相反，在第2天和第3天发现循环T2淋巴细胞（活性玫瑰花结形成细胞）显著增加（50 - 100%），随后这些“分化”细胞迅速减少。T2淋巴细胞的增加在皮肤试验阳性的受试者中出现得更早。这些变化与E玫瑰花结、有丝分裂原刺激、外周白细胞迁移抑制以及抗原存在下的转化相关。EA-IgG玫瑰花结和ADCC也有类似变化。这些结果可能表明再次接种疫苗后有显著的非特异性细胞动员。
