蔗糖孔蛋白ScrY的L3环定点诱变导致底物选择性发生变化。
Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity.
作者信息
Ulmke C, Kreth J, Lengeler J W, Welte W, Schmid K
机构信息
AG Genetik, FB Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück, Germany.
出版信息
J Bacteriol. 1999 Mar;181(6):1920-3. doi: 10.1128/JB.181.6.1920-1923.1999.
Abstract
The difference in substrate selectivity of the maltodextrin (LamB) and sucrose (ScrY) porins is attributed mainly to differences in loop L3, which is supposed to constrict the lumen of the pores. We show that even a single mutation (D201Y) in loop L3 leads to a narrowing of the substrate range of ScrY to that resembling LamB. In addition, we removed the putative N-terminal coiled-coil structure of ScrY and studied the effect of this deletion on sucrose transport.
摘要
麦芽糊精（LamB）孔蛋白和蔗糖（ScrY）孔蛋白在底物选择性上的差异主要归因于环L3的差异，环L3被认为会使孔腔变窄。我们发现，即使环L3中单个突变（D201Y）也会导致ScrY的底物范围变窄，使其类似于LamB。此外，我们去除了ScrY假定的N端卷曲螺旋结构，并研究了这种缺失对蔗糖转运的影响。
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