筛选具有高突变率的大肠杆菌突变体。
Selection of a mutant of Escherichia coli which has high mutation rates.
作者信息
Helling R B
出版信息
J Bacteriol. 1968 Oct;96(4):975-80. doi: 10.1128/jb.96.4.975-980.1968.
Abstract
A mutation which causes high mutation rates in all other loci tested was induced with nitrosoguanidine and was selected through the ability of the progeny of such mutant cells to mutate to streptomycin resistance at a higher rate than the wild-type cells. This mutation (mut-2) and the Treffers' mutation (mutT1) mapped at approximately the same position to the right of leu. Specificity studies showed that the two mutations differ in rates of mutation produced.
摘要
用亚硝基胍诱导出一种在所有其他测试位点都能导致高突变率的突变，并通过这种突变细胞后代比野生型细胞以更高频率突变为链霉素抗性的能力进行筛选。这种突变（mut - 2）和特雷弗斯突变（mutT1）在leu右侧大致相同的位置进行了定位。特异性研究表明，这两种突变在产生的突变率上有所不同。
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