噬菌体P1在大肠杆菌中以及噬菌体P22在鼠伤寒沙门氏菌中对各种R因子的转导。
Transduction of various R factors by phage P1 in Escherichia coli and by phage P22 in Salmonella typhimurium.
作者信息
Watanabe T, Furuse C, Sakaizumi S
出版信息
J Bacteriol. 1968 Nov;96(5):1791-5. doi: 10.1128/jb.96.5.1791-1795.1968.
Abstract
R factors fi(+) and fi(-), with various combinations of drug-resistance markers and isolated from independent sources, were transduced by phage P1kc in Escherichia coli and by phage P22 in Salmonella typhimurium. Usually the entire R factor was transduced by P1kc in E. coli, as indicated by the absence of segregation of the drug-resistance markers from their conjugal transferability. In contrast, the patterns of segregation of the drug-resistance markers and their conjugal transferability differed considerably among various R factors after transduction by P22 in S. typhimurium. Transduction frequencies varied among R factors in both transduction systems.
摘要
具有不同耐药性标记组合且从独立来源分离得到的R因子fi(+)和fi(-)，在大肠杆菌中由噬菌体P1kc进行转导，在鼠伤寒沙门氏菌中由噬菌体P22进行转导。通常，P1kc在大肠杆菌中转导整个R因子，这表现为耐药性标记与其接合转移性之间不存在分离。相比之下，在鼠伤寒沙门氏菌中由P22转导后，不同R因子之间耐药性标记的分离模式及其接合转移性差异很大。在两个转导系统中，不同R因子的转导频率各不相同。
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