Dalgarno L, Sinsheimer R L
J Virol. 1968 Aug;2(8):822-9. doi: 10.1128/JVI.2.8.822-829.1968.
A group of temperature-sensitive mutants of phiX174 has been isolated which can go through a single, normal one-step growth cycle at 40 C but fail to form plaques at this temperature. Such mutants fail to initiate a second cycle at 40 C; however they can gain the capacity to infect at 40 C, upon incubation for 10 min in broth at 30 C. In regaining the ability to infect, the phage appear to undergo a temperature-dependent conformational alteration. The inverse process, a reversible loss of ability to infect at 40 C, is observed when such phage produced at 30 C are incubated for 2 hr at 40 C. The defect in initiation of a second cycle of infection appears to be in the injection of viral deoxyribonucleic acid. A two-step complementation test has been used to identify the cistron coding for the affected function. Such mutants are also unusually sensitive to an irreversible thermal inactivation when incubated at 40 C.
已分离出一组φX174温度敏感突变体，它们在40℃时可经历单个正常的一步生长周期，但在此温度下无法形成噬菌斑。此类突变体在40℃时无法启动第二个周期；然而，在30℃的肉汤中孵育10分钟后，它们能够获得在40℃感染的能力。在恢复感染能力时，噬菌体似乎经历了温度依赖性的构象改变。当在30℃产生的此类噬菌体在40℃孵育2小时时，会观察到相反的过程，即在40℃时感染能力的可逆丧失。第二次感染周期起始的缺陷似乎在于病毒脱氧核糖核酸的注入。已使用两步互补试验来鉴定编码受影响功能的顺反子。此类突变体在40℃孵育时对不可逆的热失活也异常敏感。
