Silver S
Proc Natl Acad Sci U S A. 1969 Mar;62(3):764-71. doi: 10.1073/pnas.62.3.764.
Magnesium-28 is accumulated by cells of Escherichia coli by a process that is temperature-dependent and inhibited by dinitrophenol and cyanide. The rate of uptake of magnesium is concentration-dependent, with a "K(m)" of about 5 x 10(-4)M in tryptone broth. There is evidence for cellular control over this rate. The magnesium accumulation system is specific, since neither potassium nor calcium (nor apparently manganese) competes with magnesium for cellular uptake. The magnesium that is accumulated by the cells can be released by treatment with toluene and partially exchanges with excess exogenous magnesium.
大肠杆菌细胞通过一个依赖温度且受二硝基苯酚和氰化物抑制的过程积累镁-28。镁的摄取速率取决于浓度,在胰蛋白胨肉汤中“米氏常数”约为5×10⁻⁴M。有证据表明细胞对该速率有调控。镁积累系统具有特异性,因为钾、钙(显然还有锰)都不会与镁竞争细胞摄取。细胞积累的镁可通过甲苯处理释放,并能与过量的外源镁部分交换。