Fisher S, Buxbaum L, Toth K, Eisenstadt E, Silver S
J Bacteriol. 1973 Mar;113(3):1373-80. doi: 10.1128/jb.113.3.1373-1380.1973.
An overnight culture of Bacillus subtilis W23 in low-manganese tryptone broth is unable to sporulate and becomes hyperactive with regard to the manganese active transport system during stationary phase. When manganese is added to cells in spent or fresh medium, the cells immediately accumulate a high proportion of the manganese available in the medium. When the hyperactive cells are diluted into broth containing 10 muM Mn(2+), high intracellular manganese levels are reached, and inhibition of ribonucleic acid and protein synthesis occurs. This inhibition is relieved when the intracellular manganese concentration declines to the nontoxic levels characteristic of cells growing in 10 muM Mn(2+). The release of the accumulated manganese is achieved by a reduction in the uptake rate for manganese while the efflux rate remains essentially constant. Inhibitors of ribonucleic acid and protein synthesis prevent the reduction of the high rate of manganese uptake and, therefore, high net concentrations of manganese are maintained in the presence of these inhibitors. The hyperactive manganese uptake system is temperature dependent and inhibited by cyanide and m-chlorophenyl carbonylcyanide hydrazone.
枯草芽孢杆菌W23在低锰胰蛋白胨肉汤中的过夜培养物无法形成芽孢，并且在稳定期对于锰的主动转运系统变得过度活跃。当向用过的或新鲜培养基中的细胞添加锰时，细胞会立即积累培养基中大部分可用的锰。当将过度活跃的细胞稀释到含有10 μM Mn(2+)的肉汤中时，细胞内锰水平会升高，并且会发生核糖核酸和蛋白质合成的抑制。当细胞内锰浓度降至在10 μM Mn(2+)中生长的细胞特有的无毒水平时，这种抑制作用会解除。通过降低锰的摄取速率来实现积累的锰的释放，而流出速率基本保持恒定。核糖核酸和蛋白质合成抑制剂会阻止锰高摄取速率的降低，因此，在存在这些抑制剂的情况下，锰会保持高净浓度。过度活跃的锰摄取系统依赖于温度，并受到氰化物和间氯苯基羰基氰化物腙的抑制。
