Gelman B B, Michaelson I A
J Toxicol Environ Health. 1979 Jul;5(4):671-82. doi: 10.1080/15287397909529779.
Neonatal rats were given aqueous lead acetate intragastrically from d 2--20 of life at doses of 0, 25, 75, and 225 mg Pb/kg.d. Blood Pb concentrations on d 21 were (mean +/- SE) 27 +/- 4 (control), 150 +/- 26, 263 +/- 63, and 518 +/- 97 microgram/100 ml, respectively. Growth was significantly depressed only in animals given the highest dose of Pb (225 mg/kg.d). Hematocrits were significantly decreased by d21 at all doses of Pb. Malondialdehyde (MDA) formation in 750 x g (10 min) brain supernatants induced spontaneously by aerobic incubation at 37 degrees C was not altered by Pb on d 7 and 14, but a slight decrease was observed on d 21. The extent of MDA formation induced by enzymatically generated superoxide anion was not altered by Pb toxicity during the first 21 d of life. Addition of Pb to 750 x g (10 min) brain supernatants in vitro significantly decreased MDA formation at Pb concentrations of 10(-5) M and higher. These results show that the central nervous system toxicity of Pb in neonatal rats is not associated with accelerated in vitro lipid peroxidation of brain tissue.
新生大鼠在出生后第2天至第20天经口给予醋酸铅水溶液，剂量分别为0、25、75和225 mg Pb/kg·d。第21天的血铅浓度（均值±标准误）分别为27±4（对照组）、150±26、263±63和518±97 μg/100 ml。仅给予最高剂量铅（225 mg/kg·d）的动物生长明显受到抑制。在所有铅剂量组中，第21天时血细胞比容均显著降低。在37℃有氧孵育自发诱导的750×g（10分钟）脑匀浆上清液中，丙二醛（MDA）的生成在第7天和第14天时不受铅的影响，但在第21天时略有下降。在出生后的前21天内，酶促产生的超氧阴离子诱导的MDA生成程度不受铅毒性的影响。在体外，向750×g（10分钟）脑匀浆上清液中添加铅，当铅浓度达到10⁻⁵ M及更高时，显著降低了MDA的生成。这些结果表明，新生大鼠铅的中枢神经系统毒性与脑组织体外脂质过氧化加速无关。
