Gershon R K, Kondo K
Immunology. 1971 Dec;21(6):903-14.
Previous studies have shown that thymectomized lethally irradiated bone marrow grafted mice, reconstituted with thymocytes and pretreated with a large dose of sheep red blood cells (SRBC), are unable to respond to a subsequent immunizing injection of SRBC even after an inoculation of normal thymocytes. If, however, the mice are not thymocyte reconstituted prior to the pretreatment with SRBC, they can respond almost normally to an immunizing injection of SRBC if inoculated with normal thymocytes after the termination of antigen pretreatment. In the present study the immunosuppressive effect of the presence of thymocytes during the antigen pretreatment was studied by adoptively transferring the spleen cells of the antigen pretreated mice to thymus-deprived chimeras. These spleen cells not only did not co-operate with normal thymocytes in the secondary hosts, but they also prevented the co-operation of normal thymocytes with normal bone marrow derived cells. Untreated spleen cells or treated spleen cells from mice not reconstituted with thymocytes did not affect cell co-operation in the secondary hosts. The abrogation of the co-operation in the secondary host was specific in that the addition of spleen cells did not affect the anti-horse red blood cell response. If the primary host made antibody as a result of the pretreatment, the transfer of their spleen cells did not prevent antibody production in the secondary host.
先前的研究表明，经胸腺切除、致死剂量照射并移植骨髓的小鼠，用胸腺细胞重建并经大剂量绵羊红细胞（SRBC）预处理后，即使接种正常胸腺细胞，也无法对随后的SRBC免疫注射产生反应。然而，如果小鼠在SRBC预处理之前未用胸腺细胞重建，那么在抗原预处理结束后接种正常胸腺细胞时，它们几乎能对SRBC免疫注射正常反应。在本研究中，通过将抗原预处理小鼠的脾细胞过继转移到无胸腺嵌合体中，研究了抗原预处理期间胸腺细胞存在的免疫抑制作用。这些脾细胞不仅在次级宿主中不与正常胸腺细胞协同作用，而且还阻止正常胸腺细胞与正常骨髓来源细胞的协同作用。未用胸腺细胞重建的小鼠的未处理脾细胞或处理过的脾细胞，不会影响次级宿主中的细胞协同作用。次级宿主中协同作用的消除具有特异性，因为添加脾细胞不会影响抗马红细胞反应。如果初级宿主因预处理而产生抗体，其脾细胞的转移不会阻止次级宿主中的抗体产生。
