Toji L, Cohen S S
J Bacteriol. 1970 Aug;103(2):323-8. doi: 10.1128/jb.103.2.323-328.1970.
2',3'-Dideoxyadenosine was previously shown to be lethal to Escherichia coli and to inhibit deoxyribonucleic acid (DNA) synthesis irreversibly in this organism. It was also shown that triphosphate of this analogue terminates DNA chains in an in vitro system. Data presented here show that the nucleoside is relatively insensitive to E. coli adenosine deaminase and is converted intracellularly into the dideoxynucleotide, including the triphosphate. Thymine nucleotide pools were not reduced in inhibited bacteria, nor did preformed DNA break down. Some adenine was liberated from the dideoxyadenosine on incubation, and the latter was incorporated into ribonucleic acid. Nevertheless, about 4,000 molecules of the dideoxynucleoside were incorporated into DNA per cell. The dideoxynucleotide occurred in DNA chains in a terminal position, liberated selectively by venom phosphodiesterase. The possible nature of the lethal event is discussed.
2',3'-双脱氧腺苷先前已被证明对大肠杆菌具有致死性，并能在该生物体中不可逆地抑制脱氧核糖核酸（DNA）合成。还表明该类似物的三磷酸酯在体外系统中会终止DNA链。此处呈现的数据表明，该核苷对大肠杆菌腺苷脱氨酶相对不敏感，并在细胞内转化为双脱氧核苷酸，包括三磷酸酯。在受抑制的细菌中，胸腺嘧啶核苷酸池并未减少，预先形成的DNA也没有分解。在孵育时，一些腺嘌呤从双脱氧腺苷中释放出来，并且后者被掺入核糖核酸中。然而，每个细胞约有4000个双脱氧核苷分子被掺入DNA中。双脱氧核苷酸出现在DNA链的末端位置，可被蛇毒磷酸二酯酶选择性地释放出来。文中讨论了致死事件的可能性质。
