Molloy P L, Powell B C, Gregg K, Barone E D, Rogers G E
Nucleic Acids Res. 1982 Oct 11;10(19):6007-21. doi: 10.1093/nar/10.19.6007.
A genomic clone containing sequences of five feather keratin genes has been isolated using cDNA to chicken embryonic feather keratin mRNA as a probe. The clone probably represents part of a longer cluster of tandemly spaced genes; the genes are evenly spaced with a centre to centre separations of 3.3 kb and are transcribed from the same DNA strand, suggesting that the cluster has arisen by a series of tandem duplications. The organisation and complete sequence of the central gene has been determined. The protein encoded by the gene contains 97 amino acids and its sequence is typical of proteins of the embryonic and adult feather family. The transcript from the gene has been deduced to contain a long 3' non-coding region of 435 nucleotides and a 58 base 5' non-coding region interrupted in the gene 21 bases prior to the initiation codon by the gene's only intron of 324 bases.
利用鸡胚羽毛角蛋白mRNA的cDNA作为探针，分离出了一个包含五个羽毛角蛋白基因序列的基因组克隆。该克隆可能代表了一个更长的串联排列基因簇的一部分；这些基因间隔均匀，中心间距为3.3 kb，并且从同一条DNA链转录，这表明该基因簇是通过一系列串联重复产生的。已经确定了中心基因的结构和完整序列。该基因编码的蛋白质含有97个氨基酸，其序列是胚胎和成年羽毛家族蛋白质的典型序列。已推断该基因的转录本包含一个435个核苷酸的长3'非编码区和一个58个碱基的5'非编码区，该非编码区在起始密码子之前21个碱基处被该基因唯一的324个碱基的内含子打断。
