转运核糖核酸中一种超修饰核苷的成熟过程。
Maturation of a hypermodified nucleoside in transfer RNA.
作者信息
Agris P F, Armstrong D J, Schäfer K P, Söll D
出版信息
Nucleic Acids Res. 1975 May;2(5):691-8. doi: 10.1093/nar/2.5.691.
Abstract
E. coli C6 rel- met- cys- was cultured in a fully supplemented medium and in media lacking cysteine or methionine. tRNA isolated from the three cultures containted, respectively, a normal complement of modified nucleosides; a deficiency in thiolated nucleosides and a deficiency in methylated nucleosides. Both sulfur-deficient tRNA and methyl-deficient tRNA contained large amounts of N-6- (delta-2-isopentenyl) adenosine and small amounts of the 2-methylthio derivative. Methyl-deficient tRNA contained, in addition a large amount of a cytokinin active, differently modified nucleoside that is believed to be a sulfur derivative of N6-(delta-2-isopentenyl) adenosine. The structure of this compound is unknown. When methly-deficient tRNA and the precusor the tRNA-Tyr su3-+ A25 were enzymatically methylated in vitro, methyl groups were incorporated into derivatives of isopentenyladenosine. These results indicate that the biosynthesis of the 2-methylthio derivative of isopentenyladenosine may occur in a sequential manner, i.e., thiolation of isopentenyladenosine followed by methylation.
摘要
大肠杆菌C6 rel- met- cys- 在完全补充培养基以及缺乏半胱氨酸或甲硫氨酸的培养基中培养。从这三种培养物中分离出的tRNA分别含有正常的修饰核苷互补物；硫醇化核苷缺乏以及甲基化核苷缺乏。硫缺乏的tRNA和甲基缺乏的tRNA都含有大量的N-6-（δ-2-异戊烯基）腺苷和少量的2-甲硫基衍生物。此外，甲基缺乏的tRNA含有大量具有细胞分裂素活性、修饰不同的核苷，据信它是N6-（δ-2-异戊烯基）腺苷的硫衍生物。该化合物的结构未知。当甲基缺乏的tRNA和tRNA-Tyr su3- + A25的前体在体外进行酶促甲基化时，甲基被掺入异戊烯基腺苷的衍生物中。这些结果表明，异戊烯基腺苷的2-甲硫基衍生物的生物合成可能以顺序方式发生，即异戊烯基腺苷先硫醇化，然后甲基化。
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