Bhattacharyya P, Epstein W, Silver S
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1488-92. doi: 10.1073/pnas.68.7.1488.
Osmotically shocked Escherichia coli and membrane vesicle ghosts from E. coli cells have lost the ability to accumulate potassium by active transport. The addition of valinomycin to the membrane ghosts restores the capacity to accumulate radioactive (42)K and (86)Rb by a temperature- and energy-dependent process. Membrane vesicles prepared from mutants of E. coli altered in potassium transport show defects in the valinomycin-stimulated accumulation of (42)K that are related to the defects in the intact cells.
经渗透休克处理的大肠杆菌以及来自大肠杆菌细胞的膜泡空壳已丧失通过主动运输积累钾的能力。向膜空壳中添加缬氨霉素可通过温度和能量依赖的过程恢复积累放射性（42）K和（86）Rb的能力。从钾转运发生改变的大肠杆菌突变体制备的膜泡在缬氨霉素刺激的（42）K积累方面表现出缺陷，这些缺陷与完整细胞中的缺陷相关。
