Bhattacharyya P
J Bacteriol. 1975 Jul;123(1):123-7. doi: 10.1128/jb.123.1.123-127.1975.
Membrane vesicles isolated from cells of bacillus subtilis W23 accumulate manganese in the presence of an energy source. The artificial electron donor system ascorbate and phenazine methosulfate or reduced nicotinamide adenine dinucleotide and phenazine methosulfate can supply the energy for the uptake. D-Lactate in the presence or absence of phenazine methosulfate would not support manganese accumulation. Anaerobiosis, cyanide, m-chlorophenyl carbonylcyanide hydrozone, valinomycin, gramicidin, and p-hydroxy-mercuribenzoate inhibit the uptake. The inhibition by p-hydroxymercuribenzoate is prevented by excess dithiothreitol. Potassium fluoride or sodium arsenate has no effect on the uptake. The manganese transport system in the B. subtilis vesicles exhibits Michaelis-Menten kinetics with a Km of 13 muM and a Vmax of 1.7 nmol/min per mg (dry weight) of membranes. The uptake of manganese is specific and is not inhibited by 0.1 mM CaCL2 or Mgcl2.
从枯草芽孢杆菌W23细胞中分离出的膜泡在有能量来源的情况下会积累锰。人工电子供体系统抗坏血酸和硫酸吩嗪甲酯或还原型烟酰胺腺嘌呤二核苷酸和硫酸吩嗪甲酯可为摄取提供能量。无论有无硫酸吩嗪甲酯，D-乳酸都不能支持锰的积累。厌氧、氰化物、间氯苯基羰基氰化物腙、缬氨霉素、短杆菌肽和对羟基汞苯甲酸盐会抑制摄取。过量的二硫苏糖醇可防止对羟基汞苯甲酸盐的抑制作用。氟化钾或砷酸钠对摄取没有影响。枯草芽孢杆菌膜泡中的锰转运系统表现出米氏动力学，Km为13μM，Vmax为每毫克（干重）膜1.7 nmol/分钟。锰的摄取具有特异性，不受0.1 mM氯化钙或氯化镁的抑制。
