青枯假单胞菌的表型特征及脱氧核糖核酸同源性
Phenotypic characterization and deoxyribonucleic acid homologies of Pseudomonas solanacearum.
作者信息
Palleroni N J, Doudoroff M
出版信息
J Bacteriol. 1971 Sep;107(3):690-6. doi: 10.1128/jb.107.3.690-696.1971.
Abstract
Twenty-six strains and colony variants of Pseudomonas solanacearum belonging to four described biotypes were characterized, by using 169 phenotypic characters previously found useful in distinguishing among strains of other Pseudomonas species. Deoxyribonucleic acid (DNA) hybridization (intra- and interspecific DNA-DNA hybridizations) was performed by using the in vitro "DNA competition" technique. P. solanacearum appears to be a moderately homogeneous species, which is, at most, only remotely related to all other species of the genus studied to date. The four biotypes are not clearly distinct from one another with respect to nutritional characters or DNA homologies. Discrepancies between acid production and growth with some carbohydrates were noted. Difficulties were encountered in certain DNA competition experiments and some problems of the methodology are discussed.
摘要
利用先前发现的有助于区分其他假单胞菌属菌株的169个表型特征，对属于4个已描述生物型的26株茄科假单胞菌及其菌落变体进行了特征分析。采用体外“DNA竞争”技术进行了脱氧核糖核酸（DNA）杂交（种内和种间DNA-DNA杂交）。茄科假单胞菌似乎是一个中等程度的同质物种，与迄今为止研究的该属所有其他物种最多只有远缘关系。这4个生物型在营养特征或DNA同源性方面彼此之间没有明显区别。注意到在利用某些碳水化合物产酸与生长之间存在差异。在某些DNA竞争实验中遇到了困难，并讨论了该方法的一些问题。
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