PathoTec“快速身份识别系统”的评估
Evaluation of the PathoTec "Rapid I-D System".
作者信息
Blazevic D J, Schreckenberger P C, Matsen J M
出版信息
Appl Microbiol. 1973 Dec;26(6):886-9. doi: 10.1128/am.26.6.886-889.1973.
Abstract
The 10 biochemical test strips included in the PathoTec Rapid I-D System were evaluated for accuracy as compared to standard tests and for efficacy in identification of 193 gram-negative bacilli. The test agreement was 100% for oxidase and phenylalanine deaminase, 99% for indole, nitrate, and Voges-Proskauer, 98% for malonate, 97% for lysine decarboxylase, 90% for urease, 84% for H(2)S, and 75% for esculin hydrolysis. Most of the commonly isolated Enterobacteriaceae were identified correctly within 4 h. Errors in identification of Proteus morganii and P. rettgeri occurred because of positive H(2)S tests on the PathoTec strips with these organisms.
摘要
将PathoTec快速鉴定系统中包含的10种生化测试条与标准测试相比，评估其准确性，并评估其对193株革兰氏阴性杆菌的鉴定效力。氧化酶和苯丙氨酸脱氨酶的测试一致性为100%，吲哚、硝酸盐和Voges-Proskauer的测试一致性为99%，丙二酸盐的测试一致性为98%，赖氨酸脱羧酶的测试一致性为97%，脲酶的测试一致性为90%，H(2)S的测试一致性为84%，七叶苷水解的测试一致性为75%。大多数常见分离的肠杆菌科细菌在4小时内被正确鉴定。由于这些生物体在PathoTec测试条上的H(2)S测试呈阳性，导致摩根变形杆菌和雷氏变形杆菌的鉴定出现错误。
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Comparative study of the efficacy of seven paper-reagent strips and conventional biochemical tests in identifying gram-negative organisms.七种试纸条与传统生化检测法鉴定革兰氏阴性菌的效能比较研究
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