使用计算机数据库对一种用于发酵革兰氏阴性杆菌的小型“传统”鉴定系统进行评估。

Evaluation of a small "conventional" identification system for fermentative gramnegative rods using a computerized data base.

作者信息

Siegrist H H, Altwegg M, Wenger A, Isenrich H, von Graevenitz A

机构信息

Institut de microbiologie, CHUV, Lausanne, Switzerland.

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Oct;266(3-4):370-8. doi: 10.1016/s0176-6724(87)80217-1.

DOI:10.1016/s0176-6724(87)80217-1

PMID:3326369

Abstract

A small "conventional" identification system consisting of six tubed media totalling eleven reactions was used to identify 467 strains of Enterobacteriaceae, 15 strains of Aeromonas sp., and 3 strains of Plesiomonas shigelloides. The system identified 371 strains (76.5%) correctly to maximum identification (species or genus) level and an additional 13 strains (2.7%) to genus level where species level identification would have been possible. Ninety strains (18.6%) were not identified. Only 11 strains (2.3%) were misidentified. The system is inexpensive compared with commercial systems and can be used for primary identification of fermentative gramnegative bacteria in a clinical microbiology laboratory. It comes with a computerized data base in the form of an identification manual.

摘要

一个小型“传统”鉴定系统，由六种含管培养基组成，共有十一种反应，用于鉴定467株肠杆菌科细菌、15株气单胞菌属细菌和3株类志贺邻单胞菌。该系统将371株（76.5%）正确鉴定到最高鉴定（种或属）水平，另外13株（2.7%）鉴定到属水平，而在该水平本可以鉴定到种水平。90株（18.6%）未被鉴定出来。只有11株（2.3%）被错误鉴定。与商业系统相比，该系统成本较低，可用于临床微生物实验室对发酵革兰氏阴性菌的初步鉴定。它配有以鉴定手册形式存在的计算机数据库。