Lowy D R, Scolnick E M
J Virol. 1978 Jan;25(1):157-63. doi: 10.1128/JVI.25.1.157-163.1978.
Dexamethasone (3 X 10(-10) to 3 X 10(-6) M) induced foci of morphologically transformed cells in a small proportion of a mink cell line that contains the Moloney murine sarcoma viral genome (S+L-). The induction was glucocorticoid specific, since other steroids with glucocorticoid activity (prednisolone, cortisol, and aldosterone) induced foci with an efficiency that paralleled their glucocorticoid activity, and steroids lacking glucocorticoid activity (17B-estradiol, testosterone, and progesterone) failed to induce foci. Viral antigen, as measured by specific immunofluorescence, was localized to the foci. The induction of foci by dexamethasone (3 X 10(-7)) was accompanied by an approximately 10-fold increase in intracellular Moloney murine sarcoma virus-specific RNA and viral p30 antigen. Removal of dexamethasone was followed by the disappearance of foci and a decrease in viral RNA and p30. In this cell system, therefore, glucocorticoids can affect the intracellular levels of type C viral RNA and protein.
地塞米松（3×10⁻¹⁰至3×10⁻⁶M）在一小部分含有莫洛尼鼠肉瘤病毒基因组（S⁺L⁻）的貂细胞系中诱导出形态转化细胞灶。这种诱导具有糖皮质激素特异性，因为其他具有糖皮质激素活性的类固醇（泼尼松龙、皮质醇和醛固酮）诱导细胞灶的效率与其糖皮质激素活性平行，而缺乏糖皮质激素活性的类固醇（17β-雌二醇、睾酮和孕酮）则不能诱导细胞灶。通过特异性免疫荧光检测，病毒抗原定位于细胞灶。地塞米松（3×10⁻⁷）诱导细胞灶的同时，细胞内莫洛尼鼠肉瘤病毒特异性RNA和病毒p30抗原增加了约10倍。去除地塞米松后，细胞灶消失，病毒RNA和p30减少。因此，在这个细胞系统中，糖皮质激素可以影响C型病毒RNA和蛋白质的细胞内水平。
