临床实验室中分离厌氧菌的实用方法。
Practical method for isolation of anerobic bacteria in the clinical laboratory.
作者信息
Martin W J
出版信息
Appl Microbiol. 1971 Dec;22(6):1168-71. doi: 10.1128/am.22.6.1168-1171.1971.
Abstract
An inexpensive but practical and simple method is described for providing an environment suitable for the maintenance and survival of clinically significant anaerobic bacteria, consistent with the needs of a large-volume laboratory. This modification of the GasPak procedure is based on two concepts. One involved the storage of "freshly prepared" plating medium under a constant stream of carbon dioxide. The other concept was to use an anaerobe jar, continuously flushed with oxygen-free carbon dioxide, as a "holding receptacle" for the inoculated media Experience with this modification has shown that the number of isolations of anaerobic bacteria from clinical material can be increased significantly.
摘要
本文描述了一种经济实惠、实用且简单的方法，该方法能提供适合临床上重要厌氧菌生存和维持的环境，满足大型实验室的需求。这种对GasPak方法的改进基于两个概念。一个是将“新制备的”平板培养基储存在持续的二氧化碳气流下。另一个概念是使用一个不断用无氧二氧化碳冲洗的厌氧罐，作为接种培养基的“存放容器”。使用这种改进方法的经验表明，从临床材料中分离出的厌氧菌数量可显著增加。
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