用于脆弱拟杆菌鉴定的胰蛋白酶大豆胆汁-卡那霉素试验
Tryptic soy bile-kanamycin test for the indentification of Bacteroides fragilis.
作者信息
Vargo V, Korzeniowski M, Spaulding E H
出版信息
Appl Microbiol. 1974 Mar;27(3):480-3. doi: 10.1128/am.27.3.480-483.1974.
Abstract
A simple and practical test for the identification of Bacteroides fragilis is described. It utilizes two well-known properties of this species, i.e., stimulation of growth by bile and resistance to kanamycin. The test media are a tryptic-soy bile agar plate and a supplemented blood agar plate on which a kanamycin 1,000-mug/ml disk is placed. Incubation is for 24 h at 37 C in GasPak. The results of screening 190 strains, mostly clinical isolates, indicate that B. fragilis can be easily and reliably distinguished from other Bacteroides and from Fusobacterium species by its growth on tryptic-soy bile agar and resistance to kanamycin.
摘要
本文描述了一种简单实用的脆弱拟杆菌鉴定试验。该试验利用了该菌的两个众所周知的特性，即胆汁刺激生长和对卡那霉素耐药。试验培养基为胰蛋白胨大豆胆汁琼脂平板和补充血琼脂平板，在后者上放置含1000μg/ml卡那霉素的纸片。在GasPak中于37℃孵育24小时。对190株菌株（大多为临床分离株）进行筛选的结果表明，脆弱拟杆菌通过在胰蛋白胨大豆胆汁琼脂上的生长情况及其对卡那霉素的耐药性，可轻松且可靠地与其他拟杆菌属和梭杆菌属菌种区分开来。
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