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芽孢杆菌孢子对萌发化合物组合的反应。

Response of Bacillus spores to combinations of germinative compounds.

作者信息

Foerster H F, Foster J W

出版信息

J Bacteriol. 1966 Mar;91(3):1168-77. doi: 10.1128/jb.91.3.1168-1177.1966.

DOI:10.1128/jb.91.3.1168-1177.1966
PMID:4956331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC316010/
Abstract

Foerster, Harold F. (University of Texas, Austin), and J. W. Foster. Response of Bacillus spores to combinations of germinative compounds. J. Bacteriol. 91:1168-1177. 1966.-Spores of 21 strains of Bacillus megaterium and 25 other strains representing 13 species of Bacillus were produced under standardized conditions. The germination of a washed spore suspension of each strain was measured as a response to various combinations of 30 different germinative compounds. The strains were first typed with respect to their response to "primary" germination compounds, i.e., glucose, l-alanine, inosine, and l-alanine-inosine mixture, and also Na(+) and K(+). The second stage was the determination of the response to various organic and inorganic anions and cations, each strain being supplied with the "primary" compounds best for it. Marked differences in germination patterns were observed among species and strains of the same species. No relation to established taxonomic lines was evident. A nonspecific requirement for ions was found for all strains, but not all ions were effective. A striking degree of interchangeability of germinative chemicals was found. "Fractional germination" was very common. A mixture of l-alanine and inosine and various ions was the best germinative solution for most strains. Some anomalous germination patterns were encountered. Those studied included a strain whose cells lysed spontaneously upon germination and other strains for which l-leucine had striking germinative powers.

摘要

福斯特,哈罗德·F.(得克萨斯大学奥斯汀分校),以及J.W.福斯特。巨大芽孢杆菌孢子对萌发化合物组合的反应。《细菌学杂志》91:1168 - 1177。1966年。——在标准化条件下制备了21株巨大芽孢杆菌菌株以及代表芽孢杆菌属13个种的其他25株菌株的孢子。测定了每种菌株的洗涤孢子悬液对30种不同萌发化合物各种组合的萌发情况。首先根据菌株对“主要”萌发化合物的反应进行分类,即葡萄糖、L - 丙氨酸、肌苷以及L - 丙氨酸 - 肌苷混合物,还有Na⁺和K⁺。第二阶段是测定对各种有机和无机阴离子及阳离子的反应,每种菌株都提供最适合它的“主要”化合物。在不同种以及同种的不同菌株之间观察到了显著的萌发模式差异。与已确立的分类谱系没有明显关系。发现所有菌株对离子都有非特异性需求,但并非所有离子都有效。发现萌发化学物质具有显著程度的互换性。“部分萌发”非常普遍。对于大多数菌株来说,L - 丙氨酸和肌苷以及各种离子的混合物是最佳的萌发溶液。遇到了一些异常的萌发模式。所研究的包括一株在萌发时细胞自发裂解的菌株以及其他一些L - 亮氨酸具有显著萌发能力的菌株。

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