Youmans A S, Youmans G P
J Bacteriol. 1966 Jun;91(6):2146-54. doi: 10.1128/jb.91.6.2146-2154.1966.
Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Myobacterium tuberculosis. J. Bacteriol. 91:2146-2154. 1966.-The ribosomal fraction of the attenuated strain, H37Ra, of Mycobacterium tuberculosis was treated with trypsin alone, ethylenediaminetetraacetic acid (EDTA) alone, EDTA and pancreatic ribonuclease, or with trypsin and ribonuclease. After each of these treatments, the ribosomal fractions were injected intraperitoneally into male CF-1 mice to test their capacity to produce an immune response to infection with virulent tubercle bacilli, strain H37Rv. Removal of protein with trypsin left the immunogenicity unchanged; EDTA alone reduced immunogenicity in the smaller vaccinating doses; EDTA plus ribonuclease reduced the immunogenicity by approximately 50% in the highest (1.0 mg) vaccinating dose; ribonuclease alone, after treatment with trypsin, reduced immunogenicity also approximately 50%. A crude mycobacterial ribonucleic acid (RNA) was prepared by extraction of the ribosomal fraction with alcohol. This RNA preparation was as effective in producing an immune response as the ribosomal fraction from which it was prepared, unless the RNA was partially or completely degraded during the preparation. The effect of ribonuclease on the immunogenicity of the RNA was similar to that obtained with the ribosomal fractions, except that ribonuclease completely destroyed the immunogenicity of a partially degraded RNA. RNA appears to be an essential part of an immunizing substance in attenuated tubercle bacilli, which produces a high degree of immunity in mice; 50 mug (dry weight) will protect approximately 80% of the mice, and as little as 0.5 mug will protect approximately 30% of the mice. Mycobacterial RNA not incorporated in Freund's incomplete adjuvant was nonimmunogenic. Yeast RNA incorporated in Freund's incomplete adjuvant was not immunogenic.
尤曼斯，安妮·S.（伊利诺伊州芝加哥西北大学医学院），以及盖伊·P.尤曼斯。胰蛋白酶和核糖核酸酶对从结核分枝杆菌分离的核糖体和核糖核酸免疫原活性的影响。《细菌学杂志》91:2146 - 2154。1966年。——结核分枝杆菌减毒株H37Ra的核糖体部分分别用胰蛋白酶、乙二胺四乙酸（EDTA）、EDTA和胰核糖核酸酶或胰蛋白酶和核糖核酸酶处理。在每种处理之后，将核糖体部分腹腔注射到雄性CF - 1小鼠体内，以测试它们对强毒株结核杆菌H37Rv感染产生免疫反应的能力。用胰蛋白酶去除蛋白质后免疫原性不变；单独使用EDTA在较小接种剂量时降低免疫原性；EDTA加核糖核酸酶在最高（1.0毫克）接种剂量时使免疫原性降低约50%；单独的核糖核酸酶在经胰蛋白酶处理后也使免疫原性降低约50%。通过用酒精提取核糖体部分制备了粗制的分枝杆菌核糖核酸（RNA）。这种RNA制剂在产生免疫反应方面与制备它的核糖体部分一样有效，除非RNA在制备过程中部分或完全降解。核糖核酸酶对RNA免疫原性的影响与对核糖体部分的影响相似，只是核糖核酸酶完全破坏了部分降解RNA的免疫原性。RNA似乎是减毒结核杆菌中一种免疫物质的重要组成部分，它能在小鼠中产生高度免疫力；50微克（干重）能保护约80%的小鼠，低至0.5微克能保护约30%的小鼠。未掺入弗氏不完全佐剂的分枝杆菌RNA无免疫原性。掺入弗氏不完全佐剂的酵母RNA无免疫原性。
