Merz G S, Wilson J B
J Bacteriol. 1966 Jun;91(6):2356-61. doi: 10.1128/jb.91.6.2356-2361.1966.
Merz, George S. (University of Wisconsin, Madison), and Joe B. Wilson. Spontaneous mutation and recombination among brucellaphages. J. Bacteriol. 91:2356-2361. 1966.-Two plaque morphology variants, as seen on Brucella abortus 544A, termed c (for clear plaque) and lc (for late-clearing plaque) were isolated from stocks of wild-type brucellaphage and from colonies of B. abortus 544A which had undergone an alteration in colonial morphology associated with the establishment of the phage carrier state. Single-burst experiments showed that the phage variants arise by spontaneous mutation of the wild-type phage during its replication on B. abortus strain R19. Two-factor crosses of independently occurring c mutant phages showed the presence of wild-type recombinants among the progeny. Control experiments showed that there are no strong selective forces against either wild-type or c mutant phage inherent in the cross-procedure. Other control experiments ruled out the possibility that wild-type phage in the cross-progeny resulted from either back mutation of the c mutants or the presence of wild-type phage among the input c mutants.
默茨，乔治·S.（威斯康星大学麦迪逊分校），以及乔·B. 威尔逊。布鲁氏菌噬菌体间的自发突变与重组。《细菌学杂志》91:2356 - 2361。1966年。——从野生型布鲁氏菌噬菌体菌液以及流产布鲁氏菌544A菌落中分离出两种在流产布鲁氏菌544A上形成的噬菌斑形态变异体，分别称为c（透明噬菌斑）和lc（后期透明噬菌斑），其中流产布鲁氏菌544A菌落的菌落形态发生了改变，与噬菌体携带状态的建立有关。单步生长实验表明，这些噬菌体变异体是野生型噬菌体在流产布鲁氏菌R19菌株上复制过程中自发突变产生的。独立出现的c突变噬菌体的双因子杂交实验表明，子代中存在野生型重组体。对照实验表明，杂交过程中不存在针对野生型或c突变噬菌体的强大选择压力。其他对照实验排除了杂交子代中的野生型噬菌体是由c突变体的回复突变或输入的c突变体中存在野生型噬菌体导致的可能性。
