Rebers P A, Heddleston K L, Rhoades K R
J Bacteriol. 1967 Jan;93(1):7-14. doi: 10.1128/jb.93.1.7-14.1967.
A heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of Pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. The original bacterial culture had been obtained from a bison that died of hemorrhagic septicemia, an infectious disease of cattle and buffalo. Injection of fractional milligram amounts of the antigen into mice, rabbits, and calves produced toxic reactions which frequently resulted in death of the host. The surviving animals demonstrated a high degree of immunity to challenge with live, virulent organisms. Two injections with 15 mug of the antigen produced a high degree of immunity in mice without the development of any signs of toxicity. The gross chemical composition and toxicity of the antigen were similar to those reported for endotoxins obtained by the Boivin or Westphal procedure. Although strong serological cross-reactions were obtained in Ouchterlony plates between the 105,000 x g antigens from the bison strain and an avian strain with antisera to these strains, these antisera agglutinated only the bacterial cells of the homologous strain. The active immunity obtained in mice by the injection of the 105,000 x g antigens of each strain was specific and could be correlated with the agglutination tests.
通过用冷的、甲醛化盐水提取，并在105,000×g下离心，从一株有毒力的、有荚膜的多杀巴斯德菌菌株中分离出一种热稳定的、颗粒状的脂多糖-蛋白质抗原复合物。最初的细菌培养物取自一头死于出血性败血症的野牛，出血性败血症是牛和水牛的一种传染病。将几毫克的抗原注射到小鼠、兔子和小牛体内会产生毒性反应，这常常导致宿主死亡。存活下来的动物对用活的、有毒力的微生物进行攻击表现出高度的免疫力。用15微克抗原进行两次注射可使小鼠产生高度免疫力，且未出现任何毒性迹象。该抗原的总体化学成分和毒性与通过博伊文或韦斯特法尔程序获得的内毒素报道的相似。尽管在奥克特洛尼平板上，来自野牛菌株的105,000×g抗原与一株禽菌株以及针对这些菌株的抗血清之间产生了强烈的血清学交叉反应，但这些抗血清仅能凝集同源菌株的细菌细胞。通过注射各菌株的105,000×g抗原在小鼠中获得的主动免疫是特异性的，并且可以与凝集试验相关联。
