通过氧化增强人补体第二成分的溶血活性。
Enharncement of the hemolytic activity of the second component of human complement by oxidation.
作者信息
Polley M J, Müller-Eberhard H J
出版信息
J Exp Med. 1967 Dec 1;126(6):1013-25. doi: 10.1084/jem.126.6.1013.
Abstract
A method has been described for the chemical modification of human C'2 which results in a pronounced enhancement of its hemolytic activity and a marked increase in the stability of the intermediate complex EAC'1a,4,2a prepared with the modified C'2. Both effects are fully explained by the observed increase in activity and stability of the C'3 converting enzyme, C'4,2a, following its generation with modified C'2. Evidence has been presented in support of the hypothesis that the modification resulting from treatment of C'2 with a critical concentration of iodine consists of oxidation of one or more sulfhydryl group within the molecule.
摘要
已描述了一种对人补体C2进行化学修饰的方法，该方法可显著增强其溶血活性，并使由修饰后的C2制备的中间复合物EAC1a,4,2a的稳定性显著提高。在用修饰后的C2生成C3转化酶C4,2a后，其活性和稳定性的增加充分解释了这两种效应。已有证据支持这样的假说，即在用临界浓度的碘处理C2时产生的修饰是由分子内一个或多个巯基的氧化所致。
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